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Santucci L, Candiano G, Petretto A, et al. From hundreds to thousands: Widening the normal human Urinome. Data Brief. 2014;1:25-8doi: 10.1016/j.dib.2014.08.006.
Santucci, L., Candiano, G., Petretto, A., Bruschi, M., Lavarello, C., Inglese, E., Giorgio Righetti, P., & Marco Ghiggeri, G. (2014). From hundreds to thousands: Widening the normal human Urinome. Data in brief, 125-8. https://doi.org/10.1016/j.dib.2014.08.006
Santucci, Laura, et al. "From hundreds to thousands: Widening the normal human Urinome." Data in brief vol. 1 (2014): 25-8. doi: https://doi.org/10.1016/j.dib.2014.08.006
Santucci L, Candiano G, Petretto A, Bruschi M, Lavarello C, Inglese E, Giorgio Righetti P, Marco Ghiggeri G. From hundreds to thousands: Widening the normal human Urinome. Data Brief. 2014 Aug 22;1:25-8. doi: 10.1016/j.dib.2014.08.006. eCollection 2014 Dec. PMID: 26217681; PMCID: PMC4459867.
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Data Brief. 2014 Aug 22;1:25-8. doi: 10.1016/j.dib.2014.08.006. eCollection 2014 Dec.

From hundreds to thousands: Widening the normal human Urinome.

Data in brief

Laura Santucci, Giovanni Candiano, Andrea Petretto, Maurizio Bruschi, Chiara Lavarello, Elvira Inglese, Pier Giorgio Righetti, Gian Marco Ghiggeri

Affiliations

  1. Nephrology, Dialysis, Transplantation Unit and Laboratory on Pathophysiology of Uremia, Istituto Giannina Gaslini, 16148 Genova, Italy.
  2. Laboratory of Mass Spectrometry - Core Facility, Istituto Giannina Gaslini, 16148 Genova, Italy.
  3. Politecnico di Milano, Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Via Mancinelli 7, 20131 Milano, Italy.

PMID: 26217681 PMCID: PMC4459867 DOI: 10.1016/j.dib.2014.08.006

Abstract

The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name "From hundreds to thousands: widening the normal human Urinome". The material supplied to Chorus Progect.org includes technical MS spectra data only.

Keywords: Combinatorial peptide ligand libraries; Mass spectrometry; Urinary proteome; Vesicles

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