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Koellisch U, Laustsen C, Nørlinger TS, et al. Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate. Physiol Rep. 2015;3(8)doi: 10.14814/phy2.12474.
Koellisch, U., Laustsen, C., Nørlinger, T. S., Østergaard, J. A., Flyvbjerg, A., Gringeri, C. V., Menzel, M. I., Schulte, R. F., Haase, A., & Stødkilde-Jørgensen, H. (2015). Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate. Physiological reports, 3(8), . https://doi.org/10.14814/phy2.12474
Koellisch, Ulrich, et al. "Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate." Physiological reports vol. 3,8 (2015). doi: https://doi.org/10.14814/phy2.12474
Koellisch U, Laustsen C, Nørlinger TS, Østergaard JA, Flyvbjerg A, Gringeri CV, Menzel MI, Schulte RF, Haase A, Stødkilde-Jørgensen H. Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate. Physiol Rep. 2015 Aug;3(8). doi: 10.14814/phy2.12474. PMID: 26272734; PMCID: PMC4562560.
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Physiol Rep. 2015 Aug;3(8). doi: 10.14814/phy2.12474.

Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate.

Physiological reports

Ulrich Koellisch, Christoffer Laustsen, Thomas S Nørlinger, Jakob Appel Østergaard, Allan Flyvbjerg, Concetta V Gringeri, Marion I Menzel, Rolf F Schulte, Axel Haase, Hans Stødkilde-Jørgensen

Affiliations

  1. Institute of Medical Engineering, Technische Universität München, Munich, Germany [email protected].
  2. Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
  3. Department of Clinical Medicine, Aarhus University, Aarhus, Denmark Department of Endocrinology and Internal medicine, Aarhus University Hospital, Aarhus, Denmark Danish Diabetes Academy, Aarhus, Denmark.
  4. Department of Clinical Medicine, Aarhus University, Aarhus, Denmark Department of Endocrinology and Internal medicine, Aarhus University Hospital, Aarhus, Denmark.
  5. Nuklearmedizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  6. GE Global Research, Munich, Germany.
  7. Institute of Medical Engineering, Technische Universität München, Munich, Germany.

PMID: 26272734 PMCID: PMC4562560 DOI: 10.14814/phy2.12474

Abstract

In the metabolism of acetate several enzymes are involved, which play an important role in free fatty acid oxidation. Fatty acid metabolism is altered in diabetes patients and therefore acetate might serve as a marker for pathological changes in the fuel selection of cells, as these changes occur in diabetes patients. Acetylcarnitine is a metabolic product of acetate, which enables its transport into the mitochondria for energy production. This study investigates whether the ratio of acetylcarnitine to acetate, measured by noninvasive hyperpolarized [1-(13)C]acetate magnetic resonance spectroscopy, could serve as a marker for myocardial, hepatic, and renal metabolic changes in rats with Streptozotocin (STZ)-induced diabetes in vivo. We demonstrate that the conversion of acetate to acetylcarnitine could be detected and quantified in all three organs of interest. More interestingly, we found that the hyperpolarized acetylcarnitine to acetate ratio was independent of blood glucose levels and prolonged hyperglycemia following diabetes induction in a type-1 diabetes model.

© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Keywords: Acetate; diabetes mellitus; hyperpolarization

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