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Guilhon CC, Abdul Wahab IR, Boylan F, et al. Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds. Evid Based Complement Alternat Med. 2015;2015:915927doi: 10.1155/2015/915927.
Guilhon, C. C., Abdul Wahab, I. R., Boylan, F., & Fernandes, P. D. (2015). Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds. Evidence-based complementary and alternative medicine : eCAM, 2015915927. https://doi.org/10.1155/2015/915927
Guilhon, Carolina Carvalho, et al. "Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds." Evidence-based complementary and alternative medicine : eCAM vol. 2015 (2015): 915927. doi: https://doi.org/10.1155/2015/915927
Guilhon CC, Abdul Wahab IR, Boylan F, Fernandes PD. Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds. Evid Based Complement Alternat Med. 2015;2015:915927. doi: 10.1155/2015/915927. Epub 2015 Jul 27. PMID: 26273315; PMCID: PMC4530238.
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Evid Based Complement Alternat Med. 2015;2015:915927. doi: 10.1155/2015/915927. Epub 2015 Jul 27.

Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds.

Evidence-based complementary and alternative medicine : eCAM

Carolina Carvalho Guilhon, Ikarastika Rahayu Abdul Wahab, Fabio Boylan, Patricia Dias Fernandes

Affiliations

  1. Federal University of Rio de Janeiro, Institute of Biomedical Science, Avenida Carlos Chagas Filho 373, CCS Building, 21941-902 Rio de Janeiro, RJ, Brazil.
  2. Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, 15400 Kota Bharu, Kelantan, Malaysia.
  3. School of Pharmacy and Pharmaceutical Sciences and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.

PMID: 26273315 PMCID: PMC4530238 DOI: 10.1155/2015/915927

Abstract

Pereskia bleo (Kunth) DC. (Cactaceae) is a plant commonly used in popular medicine in Malaysia. In this work, we evaluate the antinociceptive effect of P. bleo leaf extracts and isolated compounds in central antinociceptive model. Ethanol extract (E), hexane (H), ethyl acetate (EA), or butanol (B) fractions (30, 50, or 100 mg/kg, p.o.), sitosterol (from hexane) and vitexin (from ethyl acetate), were administered to mice. Antinociceptive effect was evaluated in the hot plate and capsaicin- or glutamate-induced licking models. Morphine (1 mg/kg, p.o.) was used as reference drug. Naloxone (1 mg/kg, i.p.), atropine (1 mg/kg, i.p.), and L-nitro arginine methyl ester (L-NAME, 3 mg/kg, i.p.) were administered 30 min earlier (100 mg/kg, p.o.) in order to evaluate the mechanism of the antinociceptive action. Higher dose of B developed an effect significantly superior to morphine-treated group. Naloxone prevented the antinociceptive effect of all fractions. L-NAME demonstrated effect against E, EA, and B. In all fractions, sitosterol and vitexin reduced the licking time after capsaicin injection. Glutamate-induced licking response was blocked by H, EA, and B. Our results indicate that Pereskia bleo fractions, sitosterol and vitexin, possessed a central antinociceptive effect. Part of this effect is mediated by opioid receptors and nitrergic pathway.

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