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Serricchio M, Schmid AW, Steinmann ME, et al. Flagellar membranes are rich in raft-forming phospholipids. Biol Open. 2015;4(9):1143-53doi: 10.1242/bio.011957.
Serricchio, M., Schmid, A. W., Steinmann, M. E., Sigel, E., Rauch, M., Julkowska, D., Bonnefoy, S., Fort, C., Bastin, P., & Bütikofer, P. (2015). Flagellar membranes are rich in raft-forming phospholipids. Biology open, 4(9), 1143-53. https://doi.org/10.1242/bio.011957
Serricchio, Mauro, et al. "Flagellar membranes are rich in raft-forming phospholipids." Biology open vol. 4,9 (2015): 1143-53. doi: https://doi.org/10.1242/bio.011957
Serricchio M, Schmid AW, Steinmann ME, Sigel E, Rauch M, Julkowska D, Bonnefoy S, Fort C, Bastin P, Bütikofer P. Flagellar membranes are rich in raft-forming phospholipids. Biol Open. 2015 Aug 14;4(9):1143-53. doi: 10.1242/bio.011957. PMID: 26276100; PMCID: PMC4582118.
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Biol Open. 2015 Aug 14;4(9):1143-53. doi: 10.1242/bio.011957.

Flagellar membranes are rich in raft-forming phospholipids.

Biology open

Mauro Serricchio, Adrien W Schmid, Michael E Steinmann, Erwin Sigel, Monika Rauch, Daria Julkowska, Serge Bonnefoy, Cécile Fort, Philippe Bastin, Peter Bütikofer

Affiliations

  1. Institute of Biochemistry & Molecular Medicine, University of Bern, Bern 3012, Switzerland [email protected] [email protected].
  2. Proteomics Core Facility, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
  3. Institute of Biochemistry & Molecular Medicine, University of Bern, Bern 3012, Switzerland.
  4. Trypanosome Cell Biology Unit, Pasteur Institute and INSERM U1201, Paris 75015, France.

PMID: 26276100 PMCID: PMC4582118 DOI: 10.1242/bio.011957

Abstract

The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.

© 2015. Published by The Company of Biologists Ltd.

Keywords: Flagella; Lipid rafts; Mass spectrometry; Membrane lipids; Sphingolipids; Trypanosoma brucei

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