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Lee HM, Joo BS, Lee CH, et al. Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes. J Menopausal Med. 2015;21(2):93-103doi: 10.6118/jmm.2015.21.2.93.
Lee, H. M., Joo, B. S., Lee, C. H., Kim, H. Y., Ock, J. H., & Lee, Y. S. (2015). Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes. Journal of menopausal medicine, 21(2), 93-103. https://doi.org/10.6118/jmm.2015.21.2.93
Lee, Hye Min, et al. "Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes." Journal of menopausal medicine vol. 21,2 (2015): 93-103. doi: https://doi.org/10.6118/jmm.2015.21.2.93
Lee HM, Joo BS, Lee CH, Kim HY, Ock JH, Lee YS. Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes. J Menopausal Med. 2015 Aug;21(2):93-103. doi: 10.6118/jmm.2015.21.2.93. Epub 2015 Aug 28. PMID: 26357647; PMCID: PMC4561747.
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J Menopausal Med. 2015 Aug;21(2):93-103. doi: 10.6118/jmm.2015.21.2.93. Epub 2015 Aug 28.

Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes.

Journal of menopausal medicine

Hye Min Lee, Bo Sun Joo, Chang Hoon Lee, Heung Yeol Kim, Ji Hoon Ock, Young Seok Lee

Affiliations

  1. Department of Molecular Biology, Natural Science College, Pusan National University, Busan, Korea.
  2. Research Center for Anti-Aging Technology Development, Pusan National University, Busan, Korea.
  3. Department of Obstetrics and Gynecology, Kosin University Hospital, Busan, Korea.
  4. Department of Obstetrics and Gynecology, Busan Adventist Hospital, Busan, Korea.
  5. Department of Pediatric Cardiology, Dona-A University Hospital, Busan, Korea.

PMID: 26357647 PMCID: PMC4561747 DOI: 10.6118/jmm.2015.21.2.93

Abstract

OBJECTIVES: Glucagon-like peptide-1 (GLP-1) is an intestinally secreted hormone and it plays an important role in the regulation of glucose homeostasis. However, the possible role of GLP-1 in the differentiation of adipose-derived stem cells (ADSCs) remains unknown. Therefore this study investigated the effect of GLP-1 on the differentiation of ADSCs into osteoblasts and adipocytes.

METHODS: ADSCs were isolated from human adipose tissues of the abdomens, cultured and characterized by flow cytometry and multi-lineage potential assay. ADSCs were induced in osteogenic and adipogenic media treated with two different doses (10 and 100 nM) of GLP-1, and then the effect of GLP-1 on differentiation of ADSCs into osteoblast and adipocyte was examined. The signaling pathway involved in these processes was also examined.

RESULTS: Isolated human ADSCs expressed mesenchymal stem cell (MSC) specific markers as well as GLP-1 receptor (GLP-1R) proteins. They also showed multiple-lineage potential of MSC. GLP-1 was upregulated the activity and mRNA expression of osteoblast-specific marker, alkaline phosphatase and the mineralization of calcium. In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2). This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).

CONCLUSION: This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation. The ERK signaling pathway seems to be involved in these differentiation processes mediated by GLP-1.

Keywords: Adipocytes; Adipogenesis; Adipose tissue; Cell differentiation; Glucagon-like peptide 1; Osteogenesis

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