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Khanmohammadi M, Waagepetersen RP, Sporring J. Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM). Front Neuroanat. 2015;9:116doi: 10.3389/fnana.2015.00116.
Khanmohammadi, M., Waagepetersen, R. P., & Sporring, J. (2015). Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM). Frontiers in neuroanatomy, 9116. https://doi.org/10.3389/fnana.2015.00116
Khanmohammadi, Mahdieh, et al. "Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)." Frontiers in neuroanatomy vol. 9 (2015): 116. doi: https://doi.org/10.3389/fnana.2015.00116
Khanmohammadi M, Waagepetersen RP, Sporring J. Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM). Front Neuroanat. 2015 Sep 01;9:116. doi: 10.3389/fnana.2015.00116. eCollection 2015. PMID: 26388743; PMCID: PMC4555025.
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Front Neuroanat. 2015 Sep 01;9:116. doi: 10.3389/fnana.2015.00116. eCollection 2015.

Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM).

Frontiers in neuroanatomy

Mahdieh Khanmohammadi, Rasmus P Waagepetersen, Jon Sporring

Affiliations

  1. Computer Science Department, University of Copenhagen Copenhagen, Denmark.
  2. Department of Mathematical Sciences, Aalborg University Aalborg, Denmark.

PMID: 26388743 PMCID: PMC4555025 DOI: 10.3389/fnana.2015.00116

Abstract

The spatial interactions of synaptic vesicles in synapses were assessed after a detailed characterization of size, shape, and orientation of the synaptic vesicles. We hypothesized that shape and orientation of the synaptic vesicles are influenced by their movement toward the active zone causing deviations from spherical shape and systematic trends in their orientation. We studied three-dimensional representations of synapses obtained by manual annotation of focused ion beam scanning electron microscopy (FIB-SEM) images of male mouse brain. The configurations of synaptic vesicles were regarded as marked point patterns, where the points are the centers of the vesicles, and the mark of a vesicle is given by its size, shape, and orientation characteristics. Statistics for marked point processes were employed to study spatial interactions between vesicles. We found that the synaptic vesicles in excitatory synapses appeared to be of oblate ellipsoid shape and in inhibitory synapses appeared to be of cigar ellipsoid shape, and followed a systematic pattern regarding their orientation toward the active zone. Moreover, there was strong evidence of spatial alignment in the orientations of pairs of synaptic vesicles, and of repulsion between them only in excitatory synapses, beyond that caused by their physical extent.

Keywords: K-function; mark variogram; orientation analysis; shape analysis; synaptic vesicles; three-dimensional point process

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