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Shariatpanahi M, Khodagholi F, Ashabi G, et al. Ameliorating of Memory Impairment and Apoptosis in Amyloid β-Injected Rats Via Inhibition of Nitric Oxide Synthase: Possible Participation of Autophagy. Iran J Pharm Res. 2015;14(3):811-24
Shariatpanahi, M., Khodagholi, F., Ashabi, G., Aghazadeh Khasraghi, A., Azimi, L., Abdollahi, M., Ghahremani, M. H., Ostad, S. N., Noorbakhsh, F., & Sharifzadeh, M. (2015). Ameliorating of Memory Impairment and Apoptosis in Amyloid β-Injected Rats Via Inhibition of Nitric Oxide Synthase: Possible Participation of Autophagy. Iranian journal of pharmaceutical research : IJPR, 14(3), 811-24.
Shariatpanahi, Marjan, et al. "Ameliorating of Memory Impairment and Apoptosis in Amyloid β-Injected Rats Via Inhibition of Nitric Oxide Synthase: Possible Participation of Autophagy." Iranian journal of pharmaceutical research : IJPR vol. 14,3 (2015): 811-24.
Shariatpanahi M, Khodagholi F, Ashabi G, Aghazadeh Khasraghi A, Azimi L, Abdollahi M, Ghahremani MH, Ostad SN, Noorbakhsh F, Sharifzadeh M. Ameliorating of Memory Impairment and Apoptosis in Amyloid β-Injected Rats Via Inhibition of Nitric Oxide Synthase: Possible Participation of Autophagy. Iran J Pharm Res. 2015;14(3):811-24. PMID: 26330869; PMCID: PMC4518109.
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Iran J Pharm Res. 2015;14(3):811-24.

Ameliorating of Memory Impairment and Apoptosis in Amyloid β-Injected Rats Via Inhibition of Nitric Oxide Synthase: Possible Participation of Autophagy.

Iranian journal of pharmaceutical research : IJPR

Marjan Shariatpanahi, Fariba Khodagholi, Ghorbangol Ashabi, Azar Aghazadeh Khasraghi, Leila Azimi, Mohammad Abdollahi, Mohammad Hossein Ghahremani, Seyed Nasser Ostad, Farshid Noorbakhsh, Mohammad Sharifzadeh

Affiliations

  1. Department of Toxicology and Pharmacology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  2. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ; Neuro Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  3. Department of Physiology, School of Medicine, Physiology Research center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran.
  4. Tehran University of Medical Sciences, International Campus, Tehran, Iran.
  5. Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  6. Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  7. Department of Toxicology and Pharmacology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. ; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 26330869 PMCID: PMC4518109

Abstract

It has been proposed that appearance of amyloid beta (Aβ) in hippocampus is one of the characteristic features of Alzheimer's disease (AD). The role of Nitric oxide (NO) in neurodegenerative disorders is controversy in different contexts. Here, we examined the effect of NO on spatial memory. For this purpose, we compared the effects of three different concentrations of L-NG-Nitroarginine Methyl Ester (L-NAME) as a nitric oxide synthase (NOS) inhibitor. We used Morris water maze (MWM) for evaluation of behavioral alterations. We also assessed the apoptosis and autophagy markers as two possible interfering pathways with NO signaling by western blot method. We found that in Aβ pretreated rats, intra-hippocampal injection of 1or 2 (μg/side) of L-NAME caused a significant reduction in escape latency and traveled distance comparing to Aβ-treatment group. Our molecular findings revealed that L-NAME could induce autophagy and attenuate apoptosis dose dependently. The protective role of autophagy and the deteriorative role of apoptosis is the hypothesis that can vindicate our findings. Thus using NOS inhibitors at low concentrations can be one of the therapeutic approaches in the future studies.

Keywords: Alzheimer’s disease; Apoptosis; Autophagy; L-NAME; MWM

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