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Chen C, Yang HC, Lee IS. Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification. Biomater Res. 2014;18:16doi: 10.1186/2055-7124-18-16.
Chen, C., Yang, H. C., & Lee, I. S. (2014). Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification. Biomaterials research, 1816. https://doi.org/10.1186/2055-7124-18-16
Chen, Cen, et al. "Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification." Biomaterials research vol. 18 (2014): 16. doi: https://doi.org/10.1186/2055-7124-18-16
Chen C, Yang HC, Lee IS. Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification. Biomater Res. 2014 Oct 20;18:16. doi: 10.1186/2055-7124-18-16. eCollection 2014. PMID: 26331067; PMCID: PMC4552360.
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Biomater Res. 2014 Oct 20;18:16. doi: 10.1186/2055-7124-18-16. eCollection 2014.

Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification.

Biomaterials research

Cen Chen, Hyeong Cheol Yang, In-Seop Lee

Affiliations

  1. Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018 China.
  2. Department of Dental Biomaterials Science, Seoul National University, Seoul, 110-749 Korea.
  3. Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018 China ; Institute of Natural Sciences, Yonsei University, Seoul, 120-749 Korea.

PMID: 26331067 PMCID: PMC4552360 DOI: 10.1186/2055-7124-18-16

Abstract

BACKGROUND: Immobilizing bioactive molecules and osteoconductive apatite on titanium implants have investigated direct ossification. In this study, hydroxycholesterol (HC) was immobilized with apatite on titanium through simply adsorption or sandwich-like coating. Three kinds of hydroxycholesterol were chosen to induce ossification: 20α-hydroxycholesterol (20α- HC), 22(S)-hydroxycholesterol (22(S)-HC) and 25-hydroxycholesterol (25-HC).The effects of HC/apatite coating on ossification abilities were evaluated in vitro and in vivo.

RESULTS: At 6 d, adsorbed apatite/25-HC and apatite/22(S)-HC coating exhibited some cytotoxicity, while the cell viability of apatite/20α-HC coating was similar as apatite coating. Immobilizing HC with apatite significantly enhanced the ALP activities compared with apatite coating. There was no significant difference in ALP value between adsorbed apatite/HC coating and sandwich-like apatite/HC/apatite coating. When compared with apatite coating, the mineral deposition improved by adsorbed HC with apatite at higher concentration in vivo.

CONCLUSIONS: When compared with apatite coating, immobilizing HC with apatite coating induced the ossification in vitro and in vivo.

Keywords: Apatite; Hydroxycholesterol; Ossification; Titanium

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