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Ma J, Frank MH. Isolation of Circulating Melanoma Cells. Methods Mol Biol. 2015;doi: 10.1007/7651_2015_300.
Ma, J., & Frank, M. H. (2015). Isolation of Circulating Melanoma Cells. Methods in molecular biology (Clifton, N.J.), . https://doi.org/10.1007/7651_2015_300
Ma, Jie, and Frank, Markus H. "Isolation of Circulating Melanoma Cells." Methods in molecular biology (Clifton, N.J.) vol. (2015). doi: https://doi.org/10.1007/7651_2015_300
Ma J, Frank MH. Isolation of Circulating Melanoma Cells. Methods Mol Biol. 2015 Sep 29; doi: 10.1007/7651_2015_300. Epub 2015 Sep 29. PMID: 26415609; PMCID: PMC4884170.
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Methods Mol Biol. 2015 Sep 29; doi: 10.1007/7651_2015_300. Epub 2015 Sep 29.

Isolation of Circulating Melanoma Cells.

Methods in molecular biology (Clifton, N.J.)

Jie Ma, Markus H Frank

Affiliations

  1. Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  2. Transplantation Research Program, Division of Nephrology, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA.
  3. Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. [email protected].
  4. Transplantation Research Program, Division of Nephrology, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA. [email protected].

PMID: 26415609 PMCID: PMC4884170 DOI: 10.1007/7651_2015_300

Abstract

Circulating melanoma cells (CMCs) represent critical mediators of metastatic melanoma progression. However, isolation and characterization of CMCs has been challenging due to the low frequency of these cells and the paucity of melanoma-specific cell surface markers. Herein, we describe a method for the isolation of CMCs that employs two independent markers, displays high sensitivity for CMC enrichment, and can be readily adapted to include additional molecular melanoma markers of interest. CMCs isolated by this method are enriched for ABCB5-positive melanoma stem cells, are tumorigenic in xenotransplantation assays, and can be used for phenotypical, genetic, and functional investigations of CMC biology.

Keywords: ABCB5; Circulating melanoma cells; Circulating tumor cells; Flow cytometry; Melanoma stem cells; Metastasis; Tumorigenicity; Xenotransplantation

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