Display options
Cite
Mongomake K, Doungous O, Khatabi B, et al. Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon. Springerplus. 2015;4:477doi: 10.1186/s40064-015-1272-4.
Mongomake, K., Doungous, O., Khatabi, B., & Fondong, V. N. (2015). Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon. SpringerPlus, 4477. https://doi.org/10.1186/s40064-015-1272-4
Mongomake, Kone, et al. "Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon." SpringerPlus vol. 4 (2015): 477. doi: https://doi.org/10.1186/s40064-015-1272-4
Mongomake K, Doungous O, Khatabi B, Fondong VN. Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon. Springerplus. 2015 Sep 04;4:477. doi: 10.1186/s40064-015-1272-4. eCollection 2015. PMID: 26361578; PMCID: PMC4559553.
Copy Download .nbib Format: NLM
Share it on

Springerplus. 2015 Sep 04;4:477. doi: 10.1186/s40064-015-1272-4. eCollection 2015.

Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon.

SpringerPlus

Kone Mongomake, Oumar Doungous, Behnam Khatabi, Vincent N Fondong

Affiliations

  1. Department of Biological Sciences, Delaware State University, Dover, DE 19901 USA ; Laboratory of Crop Breeding, Department of Natural Sciences, University Nangui Abrogoua, Abidjan, Côte D'Ivoire.
  2. Laboratory of Crop Breeding, Department of Natural Sciences, University Nangui Abrogoua, Abidjan, Côte D'Ivoire ; Ekona Research Center, Institute of Agronomic Research for Development, Buea, South West Region Cameroon.
  3. Department of Biological Sciences, Delaware State University, Dover, DE 19901 USA.

PMID: 26361578 PMCID: PMC4559553 DOI: 10.1186/s40064-015-1272-4

Abstract

A procedure to regenerate cassava (Manihot esculenta Crantz) cultivars from Cameroon via somatic embryogenesis (SE) was developed. Shoot apical meristems and immature leaf lobes were used as explants on Murashige and Skoog (MS) basal medium containing 33 or 50 µM of the auxins Picloram (Pic), 2,4-Dichlorophenoxyacetic acid (2,4-D), Dicamba (Dic), and α-Naphthalene acetic acid. Cultivar performance was assessed using SE and number of somatic embryos produced. Overall, the frequency of primary somatic embryogenesis (PSE) and the mean number of somatic embryos produced varied considerably with genotype, type of auxin and concentration tested. For example, cultivar (cv.) Ngan Mbada showed the best performance on MS medium supplemented with 50 µM Pic with a SE frequency of 40 % and an average number of somatic embryos of 90. The second best performance was recorded in cv. Local Red on MS medium supplemented with 33 µM 2,4-D, where the SE frequency was 40 % and an average number of somatic embryos of 60.5. Cultivar Ekona Red recorded the best performance on medium supplemented with 50 µM Pic showing a SE frequency of 47 % and an average number of somatic embryos of 45. We further examined secondary and cyclic somatic embryogenesis (SSE, CSE) and both were also observed to vary with genotype, however, both exhibited significantly higher frequencies of SE compared with PSE. SE started to decline at the fourth cycle of embryogenesis. Examination of organogenesis showed that shoot bud induction from green cotyledons varied across cultivars and benzylaminopurine was shown to outperform Thidiazuron in the ability to induce organogenesis. Furthermore, the frequencies of bud induction were identical under light and dark conditions. Finally, regenerated plants grew easily in the greenhouse with 90-100 % survival rate and did not display detectable variation in morphology.

Keywords: Cassava; Organogenesis; Plant growth regulators; Somatic embryogenesis

References

  1. Plant Mol Biol. 2004 Nov;56(4):503-16 - PubMed
  2. Annu Rev Plant Biol. 2011;62:251-72 - PubMed
  3. Nat Biotechnol. 1996 Jun;14(6):736-40 - PubMed
  4. Methods Mol Biol. 2005;286:165-76 - PubMed
  5. Plant Mol Biol. 2004 Nov;56(4):671-88 - PubMed
  6. J Integr Plant Biol. 2011 Jul;53(7):552-69 - PubMed
  7. Plant Cell Rep. 1987 Jun;6(3):248-51 - PubMed
  8. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5586-91 - PubMed
  9. Exp Cell Res. 1968 Apr;50(1):151-8 - PubMed
  10. Science. 2008 Feb 1;319(5863):607-10 - PubMed
  11. Plant Cell Rep. 1993 Apr;12(6):328-33 - PubMed
  12. Plant Cell Rep. 2011 May;30(5):779-87 - PubMed
  13. Dis Mon. 2009 Jun;55(6):336-52 - PubMed
  14. J Agric Food Chem. 2007 Sep 5;55(18):7469-76 - PubMed
  15. Sci Am. 2010 May;302(5):78-82, 84 - PubMed

Publication Types