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Ternes AP, Zemolin AP, da Cruz LC, et al. Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure. EXCLI J. 2014;13:1239-53
Ternes, A. P., Zemolin, A. P., da Cruz, L. C., da Silva, G. F., Saidelles, A. P., de Paula, M. T., Wagner, C., Golombieski, R. M., Flores, �. �. M., Picoloto, R. S., Pereira, A. B., Franco, J. L., & Posser, T. (2014). Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure. EXCLI journal, 131239-53.
Ternes, Ana Paula Lausmann, et al. "Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure." EXCLI journal vol. 13 (2014): 1239-53.
Ternes AP, Zemolin AP, da Cruz LC, da Silva GF, Saidelles AP, de Paula MT, Wagner C, Golombieski RM, Flores ÉM, Picoloto RS, Pereira AB, Franco JL, Posser T. Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure. EXCLI J. 2014 Nov 21;13:1239-53. eCollection 2014. PMID: 26417337; PMCID: PMC4464430.
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EXCLI J. 2014 Nov 21;13:1239-53. eCollection 2014.

Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure.

EXCLI journal

Ana Paula Lausmann Ternes, Ana Paula Zemolin, Litiele Cezar da Cruz, Gustavo Felipe da Silva, Ana Paula Fleig Saidelles, Mariane Trindade de Paula, Caroline Wagner, Ronaldo Medeiros Golombieski, Érico Marlon de Moraes Flores, Rochele Sogari Picoloto, Antônio Batista Pereira, Jeferson Luis Franco, Thaís Posser

Affiliations

  1. Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil.
  2. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil.
  3. Programa de Pós Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, CEP 97500-970.
  4. Universidade Federal do Pampa, Campus Caçapava do Sul, RS, CEP 96570-000 Brasil.
  5. Laboratório de Biologia Molecular de Drosophila e Sequenciamento (LabDros), Universidade Federal de Santa Maria, Santa Maria, RS, 97300 000.

PMID: 26417337 PMCID: PMC4464430

Abstract

Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an essential element, but in excess it can induce toxicity. In this study we used Drosophila melanogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38(MAPK) phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.

Keywords: Drosophila melanogaster; MAPK; Thioredoxin reductase; manganese; oxidative stress

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