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Faridi MS, Jaiswal MS, Goel SK. Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study. J Clin Diagn Res. 2015;9(7):PC04-7doi: 10.7860/JCDR/2015/12697.6152.
Faridi, M. S., Jaiswal, M. S., & Goel, S. K. (2015). Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study. Journal of clinical and diagnostic research : JCDR, 9(7), PC04-7. https://doi.org/10.7860/JCDR/2015/12697.6152
Faridi, Mohammad Shazib, et al. "Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study." Journal of clinical and diagnostic research : JCDR vol. 9,7 (2015): PC04-7. doi: https://doi.org/10.7860/JCDR/2015/12697.6152
Faridi MS, Jaiswal MS, Goel SK. Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study. J Clin Diagn Res. 2015 Jul;9(7):PC04-7. doi: 10.7860/JCDR/2015/12697.6152. Epub 2015 Jul 01. PMID: 26393162; PMCID: PMC4572993.
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J Clin Diagn Res. 2015 Jul;9(7):PC04-7. doi: 10.7860/JCDR/2015/12697.6152. Epub 2015 Jul 01.

Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study.

Journal of clinical and diagnostic research : JCDR

Mohammad Shazib Faridi, Mahabir Saran Das Jaiswal, Sudhir K Goel

Affiliations

  1. Trainee, Department of Urology, Presently at Regional Institute of Medical Sciences , Imphal, Manipur, India .
  2. Professor, Department of Surgery, Era's Lucknow Medical College & Hospital , Lucknow, U.P, India .
  3. Scientist, Petroleum Toxicology Division, Indian Institute of Toxicology Research , Lucknow, U.P, India .

PMID: 26393162 PMCID: PMC4572993 DOI: 10.7860/JCDR/2015/12697.6152

Abstract

BACKGROUND: Gastrin and cholecystokinin (CCK) receptors are trophic for various gastrointestinal malignancies. Their role in gallbladder cancer has not been widely studied.

OBJECTIVES: To identify expression of CCK-A and CCK-B receptors in the tissue and blood of patients suffering from carcinoma (CA) gallbladder and gallstone disease and to compare expression of CCK A and B receptors in the gall bladder tissue and blood of healthy individuals and patients of CA gallbladder, and gallstone diseases.

MATERIALS AND METHODS: Forty nine subjects of both genders were recruited, comprising of 22 patients of CA gall bladder, 19 cases of cholelithiasis and, 8 normal gallbladders obtained from patients operated for trauma of the biliary system or Whipple's procedure. RNA extraction and cDNA formation for CCK-A and CCK-B receptors were carried out. Real Time PCR was performed on cDNA and threshold cycle (Ct) value of each sample was obtained and ΔCt was calculated. Chi-square test for comparing two groups and ANOVA test for comparing multiple groups were applied and if p<0.05 then Dunnett-C test was performed.

OBSERVATION AND RESULTS: Both CCK-A and CCK-B receptors were expressed irrespective of its origin in all tissues and blood samples studied; be it normal, Cholelithiasis or CA gallbladder and there was no difference among them (p>0.05).

CONCLUSION: This preliminary study showed higher expression of CCK-A receptors in patients of cholelithiasis and decreased expression of CCK-A receptors in patients of CA gallbladder as compared to normal gallbladder although it did not rise to statistical significance.

Keywords: Cholecystokinin receptor; Gastrointestinal malignancies

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