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Parker SL, Adnan S, Ordóñez Meija JL, et al. An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine. Bioanalysis. 2015;7(18):2311-2319doi: 10.4155/bio.15.143.
Parker, S. L., Adnan, S., Ordóñez Meija, J. L., Paterson, D. L., Lipman, J., Roberts, J. A., & Wallis, S. C. (2015). An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine. Bioanalysis, 7(18), 2311-2319. https://doi.org/10.4155/bio.15.143
Parker, Suzanne L, et al. "An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine." Bioanalysis vol. 7,18 (2015): 2311-2319. doi: https://doi.org/10.4155/bio.15.143
Parker SL, Adnan S, Ordóñez Meija JL, Paterson DL, Lipman J, Roberts JA, Wallis SC. An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine. Bioanalysis. 2015 Sep;7(18):2311-2319. doi: 10.4155/bio.15.143. Epub 2015 Sep 23. PMID: 26395242.
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Bioanalysis. 2015 Sep;7(18):2311-2319. doi: 10.4155/bio.15.143. Epub 2015 Sep 23.

An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine.

Bioanalysis

Suzanne L Parker, Syamhanin Adnan, Jenny L Ordóñez Meija, David L Paterson, Jeffrey Lipman, Jason A Roberts, Steven C Wallis

Affiliations

  1. Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
  2. University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
  3. Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia.
  4. Department of Pharmacy, Royal Brisbane & Women's Hospital, Brisbane, Australia.

PMID: 26395242 DOI: 10.4155/bio.15.143

Abstract

BACKGROUND: With few new antibiotics available and an increased prevalence of multidrug-resistant bacteria, optimizing the effectiveness of currently available antibiotics and minimizing the potential for emerging resistance, through PK characterization, is of paramount importance.

RESULTS: Inter- and intra-assay results for ampicillin were within 5% for accuracy and 7% for precision, and for sulbactam within 7% for accuracy and 7% for precision, for both plasma and urine. The plasma Cmax was 38.4 µg/ml for ampicillin and 19.0 µg/ml for sulbactam. The urine concentrations were 668 µg/ml for ampicillin and 438 µg/ml for sulbactam.

CONCLUSION: An UHPLC-MS/MS method to simultaneously measure co-formulated ampicillin and sulbactam in plasma and urine, for use in a patient PK study, has been developed and validated.

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