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Ansari R, Buj C, Pieper M, et al. Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy. Opt Express. 2015;23(18):23217-24doi: 10.1364/OE.23.023217.
Ansari, R., Buj, C., Pieper, M., König, P., Schweikard, A., & Hüttmann, G. (2015). Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy. Optics express, 23(18), 23217-24. https://doi.org/10.1364/OE.23.023217
Ansari, Rehman, et al. "Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy." Optics express vol. 23,18 (2015): 23217-24. doi: https://doi.org/10.1364/OE.23.023217
Ansari R, Buj C, Pieper M, König P, Schweikard A, Hüttmann G. Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy. Opt Express. 2015 Sep 07;23(18):23217-24. doi: 10.1364/OE.23.023217. PMID: 26368424.
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Opt Express. 2015 Sep 07;23(18):23217-24. doi: 10.1364/OE.23.023217.

Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy.

Optics express

Rehman Ansari, Christian Buj, Mario Pieper, Peter König, Achim Schweikard, Gereon Hüttmann

PMID: 26368424 DOI: 10.1364/OE.23.023217

Abstract

Motile cilia perform a range of important mechanosensory and chemosensory functions, along with expulsion of mucus and inhaled pathogens from the lungs. Here we demonstrate that spectral domain optical coherence phase microscopy (SD-OCPM), which combines the principles of optical coherence tomography (OCT) and confocal microscopy, is particularly well-suited for characterization of both morphology and the ciliary dynamics of mouse trachea. We present micro-anatomical images of mouse trachea, where different cell types can be clearly visualized. The phase contrast, which measures the sub-nanometer changes in axial optical pathlength is used to determine the frequency and direction of cilia beatings.

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