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Kim MH, Choi YY, Lee HJ, et al. Topical application of herbal formula for the treatment of ligature-induced periodontitis. J Periodontal Implant Sci. 2015;45(4):145-51doi: 10.5051/jpis.2015.45.4.145.
Kim, M. H., Choi, Y. Y., Lee, H. J., Lee, H., Park, J. C., & Yang, W. M. (2015). Topical application of herbal formula for the treatment of ligature-induced periodontitis. Journal of periodontal & implant science, 45(4), 145-51. https://doi.org/10.5051/jpis.2015.45.4.145
Kim, Mi Hye, et al. "Topical application of herbal formula for the treatment of ligature-induced periodontitis." Journal of periodontal & implant science vol. 45,4 (2015): 145-51. doi: https://doi.org/10.5051/jpis.2015.45.4.145
Kim MH, Choi YY, Lee HJ, Lee H, Park JC, Yang WM. Topical application of herbal formula for the treatment of ligature-induced periodontitis. J Periodontal Implant Sci. 2015 Aug;45(4):145-51. doi: 10.5051/jpis.2015.45.4.145. Epub 2015 Aug 31. PMID: 26339524; PMCID: PMC4556800.
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J Periodontal Implant Sci. 2015 Aug;45(4):145-51. doi: 10.5051/jpis.2015.45.4.145. Epub 2015 Aug 31.

Topical application of herbal formula for the treatment of ligature-induced periodontitis.

Journal of periodontal & implant science

Mi Hye Kim, You Yeon Choi, Hye Ji Lee, Haesu Lee, Jung-Chul Park, Woong Mo Yang

Affiliations

  1. Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine, Seoul, Korea.
  2. Department of Periodontology, Dankook University College of Dentistry, Cheonan, Korea.

PMID: 26339524 PMCID: PMC4556800 DOI: 10.5051/jpis.2015.45.4.145

Abstract

PURPOSE: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats.

METHODS: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR.

RESULTS: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.

CONCLUSIONS: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.

Keywords: Matrix metalloproteinase 9; Medicinal herbs; Osteoclasts; Periodontitis

References

  1. Fitoterapia. 2015 Mar;101:194-200 - PubMed
  2. J Ethnopharmacol. 2010 May 27;129(2):197-202 - PubMed
  3. Dent Res J (Isfahan). 2012 Sep;9(5):505-15 - PubMed
  4. Dent Clin North Am. 2010 Jan;54(1):1-12 - PubMed
  5. Life Sci. 2009 May 22;84(21-22):745-54 - PubMed
  6. Mediators Inflamm. 1999;8(3):173-5 - PubMed
  7. Ayu. 2014 Jan;35(1):85-9 - PubMed
  8. J Periodontol. 1994 Nov;65(11):1058-66 - PubMed
  9. J Ethnopharmacol. 2014 Jun 11;154(2):380-5 - PubMed
  10. Endocrinology. 1998 Mar;139(3):1338-45 - PubMed
  11. J Clin Periodontol. 2012 Mar;39(3):284-94 - PubMed
  12. J Periodontol. 1987 Mar;58(3):147-52 - PubMed
  13. J Clin Periodontol. 2011 Feb;38(2):122-30 - PubMed
  14. J Periodontol. 1985 May;56(5):265-72 - PubMed
  15. J Periodontal Res. 1999 Oct;34(7):331-9 - PubMed
  16. J Clin Periodontol. 2003 Sep;30(9):787-94 - PubMed
  17. J Periodontol. 2001 Nov;72(11):1535-44 - PubMed
  18. Phytother Res. 2008 Jul;22(7):923-8 - PubMed
  19. Acta Odontol Scand. 2013 Mar;71(2):349-56 - PubMed
  20. J Periodontal Res. 2004 Apr;39(2):87-92 - PubMed
  21. Periodontol 2000. 1996 Feb;10:45-78 - PubMed
  22. J Med Food. 2016 Jan;19(1):98-105 - PubMed
  23. Infect Immun. 1999 Jun;67(6):2804-9 - PubMed
  24. Periodontol 2000. 1994 Jun;5:7-25 - PubMed
  25. Endocr Rev. 1999 Jun;20(3):345-57 - PubMed
  26. J Clin Periodontol. 1984 Aug;11(7):448-58 - PubMed
  27. J Periodontal Res. 2011 Oct;46(5):550-7 - PubMed
  28. Int Immunopharmacol. 2009 Feb;9(2):216-22 - PubMed
  29. Nutrients. 2014 Oct 16;6(10):4362-72 - PubMed
  30. J Microbiol Biotechnol. 2011 Aug;21(8):874-83 - PubMed
  31. J Periodontol. 1998 May;69(5):507-20 - PubMed

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