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Singec I, Knoth R, Vida I, et al. The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. Biol Open. 2015;4(10):1222-8doi: 10.1242/bio.012096.
Singec, I., Knoth, R., Vida, I., & Frotscher, M. (2015). The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. Biology open, 4(10), 1222-8. https://doi.org/10.1242/bio.012096
Singec, Ilyas, et al. "The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model." Biology open vol. 4,10 (2015): 1222-8. doi: https://doi.org/10.1242/bio.012096
Singec I, Knoth R, Vida I, Frotscher M. The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. Biol Open. 2015 Sep 04;4(10):1222-8. doi: 10.1242/bio.012096. PMID: 26340944; PMCID: PMC4610216.
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Biol Open. 2015 Sep 04;4(10):1222-8. doi: 10.1242/bio.012096.

The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model.

Biology open

Ilyas Singec, Rolf Knoth, Imre Vida, Michael Frotscher

Affiliations

  1. Institute of Anatomy and Cell Biology, Albert-Ludwigs-University Freiburg, D-79104 Freiburg, Germany Department of Neuropathology, Albert-Ludwigs-University Freiburg, D-79106 Freiburg, Germany [email protected].
  2. Department of Neuropathology, Albert-Ludwigs-University Freiburg, D-79106 Freiburg, Germany.
  3. Institute for Integrative Neuroanatomy, Charité, D-10117 Berlin, Germany.
  4. Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, D-20251 Hamburg, Germany.

PMID: 26340944 PMCID: PMC4610216 DOI: 10.1242/bio.012096

Abstract

The mouse subventricular zone (SVZ) generates large numbers of neuroblasts, which migrate in a distinct pathway, the rostral migratory stream (RMS), and replace specific interneurons in the olfactory bulb (OB). Here, we introduce an organotypic slice culture model that directly connects the RMS to the hippocampus as a new destination. RMS neuroblasts widely populate the hippocampus and undergo cellular differentiation. We demonstrate that RMS cells give rise to various neuronal subtypes and, surprisingly, to CA1 pyramidal neurons. Pyramidal neurons are typically generated before birth and are lost in various neurological disorders. Hence, this unique slice culture model enables us to investigate their postnatal genesis under defined in vitro conditions from the RMS, an unanticipated source for hippocampal pyramidal neurons.

© 2015. Published by The Company of Biologists Ltd.

Keywords: Cellular plasticity; Microenvironment; Neuronal differentiation; Organotypic slice culture; Pyramidal neuron; Rostral migratory stream

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