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Villarin BL, Cameron K, Szkolnicka D, et al. Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015). Adv Healthc Mater. 2015;4(12):1819doi: 10.1002/adhm.201570072.
Villarin, B. L., Cameron, K., Szkolnicka, D., Rashidi, H., Bates, N., Kimber, S. J., Flint, O., Forbes, S. J., Iredale, J. P., Bradley, M., & Hay, D. C. (2015). Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015). Advanced healthcare materials, 4(12), 1819. https://doi.org/10.1002/adhm.201570072
Villarin, Baltasar Lucendo, et al. "Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015)." Advanced healthcare materials vol. 4,12 (2015): 1819. doi: https://doi.org/10.1002/adhm.201570072
Villarin BL, Cameron K, Szkolnicka D, Rashidi H, Bates N, Kimber SJ, Flint O, Forbes SJ, Iredale JP, Bradley M, Hay DC. Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015). Adv Healthc Mater. 2015 Aug 26;4(12):1819. doi: 10.1002/adhm.201570072. PMID: 26308957.
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Adv Healthc Mater. 2015 Aug 26;4(12):1819. doi: 10.1002/adhm.201570072.

Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015).

Advanced healthcare materials

Baltasar Lucendo Villarin, Kate Cameron, Dagmara Szkolnicka, Hassan Rashidi, Nicola Bates, Susan J Kimber, Oliver Flint, Stuart J Forbes, John P Iredale, Mark Bradley, David C Hay

Affiliations

  1. MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
  2. Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
  3. MRC Centre for Inflammation Research, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
  4. School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3FJ, UK.

PMID: 26308957 DOI: 10.1002/adhm.201570072

Abstract

One major obstacle to the routine deployment of stem cell-derived cells is their instability in culture. On page 1820 David C. Hay and co-workers describe the use of a synthetic polymer surface. The image shows stem cell-derived hepatocytes replated on this polyurethane surface. Importantly the cells express Zonal Occludin (green stain) at the cell surface, which indicates that the cells display elements of polarization. The blue stain is DAPI, which demarks the nucleus.

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: differentiation; gene signature; hepatocytes; pluripotent stem cells; polymers

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