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Xu Z, Luo J, Yang L, et al. Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow. Int J Clin Exp Med. 2015;8(6):9320-6
Xu, Z., Luo, J., Yang, L., Wang, X., Pan, Y., Shang, Y., & Yang, J. (2015). Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow. International journal of clinical and experimental medicine, 8(6), 9320-6.
Xu, Zheng, et al. "Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow." International journal of clinical and experimental medicine vol. 8,6 (2015): 9320-6.
Xu Z, Luo J, Yang L, Wang X, Pan Y, Shang Y, Yang J. Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow. Int J Clin Exp Med. 2015 Jun 15;8(6):9320-6. eCollection 2015. PMID: 26309591; PMCID: PMC4537973.
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Int J Clin Exp Med. 2015 Jun 15;8(6):9320-6. eCollection 2015.

Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow.

International journal of clinical and experimental medicine

Zheng Xu, Jianmin Luo, Lin Yang, Xingzhe Wang, Yuxia Pan, Yintao Shang, Jingci Yang

Affiliations

  1. Department of Hematology, The Second Hospital, Hebei Medical University Shijiazhuang 050000, Hebei, P.R. China.

PMID: 26309591 PMCID: PMC4537973

Abstract

The study aim was to investigate the impacts of K562 cells towards the activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow (HMSC-bm). The in vitro co-culture of HMSC-bm and K562 cells was set as the experiment group (HMSC-bm + K562), the HMSC-bm cultured alone was set as the control group (HMSC-bm), the expressions of six interested genes and their proteins, namely MyD88, P38, NF-κB, TAB1, TLR3 and TBK1, of the Toll -like receptor signaling pathway were detected and compared, as well as the secretions of such cytokines as IL-6, IL-8, TNF-α and IFN-α in the cell supernatant, which were regulated by the Toll-like receptor pathway. The expressions of MyD88, P38, TAB1 and TLR3 of the HMSC-bm + K562 group were higher than the HMSC-bm group, while that of TBK1 was lower, and the NF-κB expression showed no significant difference between the two groups (P > 0.05). Compared with the HMSC-bm group, the supernatant of HMSC-bm + K562 group exhibited the higher secretion levels of IL-6 and IL-8, while that of IFN-α was just contrary, and the differences were significant (P < 0.05). The secretion levels of TNF-α within the two groups were not significantly different (P > 0.05). The co-culture of K562 and HMSC-bm could induce the activity changes of Toll-like receptor pathway of HMSC-bm, which was beneficial towards the proliferation of K562 cells.

Keywords: Co-culture; HMSC-bm; K562; Toll-like receptor signaling pathway

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