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Santos EC, Martinho HS, Annes K, et al. Rapid and noninvasive technique to assess the metabolomics profile of bovine embryos produced in vitro by Raman spectroscopy. Biomed Opt Express. 2015;6(8):2830-9doi: 10.1364/BOE.6.002830.
Santos, E. C., Martinho, H. S., Annes, K., Leite, R. F., & Milazzotto, M. P. (2015). Rapid and noninvasive technique to assess the metabolomics profile of bovine embryos produced in vitro by Raman spectroscopy. Biomedical optics express, 6(8), 2830-9. https://doi.org/10.1364/BOE.6.002830
Santos, E C, et al. "Rapid and noninvasive technique to assess the metabolomics profile of bovine embryos produced in vitro by Raman spectroscopy." Biomedical optics express vol. 6,8 (2015): 2830-9. doi: https://doi.org/10.1364/BOE.6.002830
Santos EC, Martinho HS, Annes K, Leite RF, Milazzotto MP. Rapid and noninvasive technique to assess the metabolomics profile of bovine embryos produced in vitro by Raman spectroscopy. Biomed Opt Express. 2015 Jul 08;6(8):2830-9. doi: 10.1364/BOE.6.002830. eCollection 2015 Aug 01. PMID: 26309747; PMCID: PMC4541511.
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Biomed Opt Express. 2015 Jul 08;6(8):2830-9. doi: 10.1364/BOE.6.002830. eCollection 2015 Aug 01.

Rapid and noninvasive technique to assess the metabolomics profile of bovine embryos produced in vitro by Raman spectroscopy.

Biomedical optics express

E C Santos, H S Martinho, K Annes, R F Leite, M P Milazzotto

Affiliations

  1. Center of Natural and Human Sciences; Universidade Federal do ABC, Brazil.

PMID: 26309747 PMCID: PMC4541511 DOI: 10.1364/BOE.6.002830

Abstract

Morphological assessments are used to select embryos with the highest implantation potential, however it is still very limited. The development of new technologies, such as Raman spectroscopy have improved quantitative and qualitative analysis, and consequently led to a better characterization of embryos and improvements on the prediction of their potential. Therefore, we propose a method based on the conventional in vitro culture system of bovine embryos, and the subsequent analysis of the culture media drops by Raman spectroscopy. Our results obtained by PCA analysis clearly showed a separation of the spectral profiles from culture media drops with and without embryos.

Keywords: (170.0170) Medical optics and biotechnology; (170.1420) Biology; (170.5660) Raman spectroscopy

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