Display options
Cite
Ng GF, Raihan IS, Azhany Y, et al. Conjunctival TGF-B Level in Primary Augmented Trabeculectomy. Open Ophthalmol J. 2015;9:136-44doi: 10.2174/1874364101509010136.
Ng, G. F., Raihan, I. S., Azhany, Y., Maraina, C. H., Banumathi, K. G., & Liza-Sharmini, T. (2015). Conjunctival TGF-B Level in Primary Augmented Trabeculectomy. The open ophthalmology journal, 9136-44. https://doi.org/10.2174/1874364101509010136
Ng, Guan Fook, et al. "Conjunctival TGF-B Level in Primary Augmented Trabeculectomy." The open ophthalmology journal vol. 9 (2015): 136-44. doi: https://doi.org/10.2174/1874364101509010136
Ng GF, Raihan IS, Azhany Y, Maraina CH, Banumathi KG, Liza-Sharmini T. Conjunctival TGF-B Level in Primary Augmented Trabeculectomy. Open Ophthalmol J. 2015 Jul 31;9:136-44. doi: 10.2174/1874364101509010136. eCollection 2015. PMID: 26401171; PMCID: PMC4578138.
Copy Download .nbib Format: NLM
Share it on

Open Ophthalmol J. 2015 Jul 31;9:136-44. doi: 10.2174/1874364101509010136. eCollection 2015.

Conjunctival TGF-B Level in Primary Augmented Trabeculectomy.

The open ophthalmology journal

Guan Fook Ng, Ishak Siti Raihan, Yaakub Azhany, Che Hussin Che Maraina, K Gurusamy Banumathi, Tajudin Liza-Sharmini

Affiliations

  1. Department of Ophthalmology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
  2. Department of Immunology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
  3. Hospital Pulau Pinang, Jalan Residensi, 10990 Georgetown, Pulau Pinang, Malaysia.

PMID: 26401171 PMCID: PMC4578138 DOI: 10.2174/1874364101509010136

Abstract

PURPOSE: To compare the levels of conjunctival transforming growth factor beta (TGF-β) between glaucoma and control patients and to determine conjunctival TGF-β levels before and 3 months after augmented primary trabeculectomy.

METHODS: Patients with primary open angle glaucoma (POAG) or primary angle closure glaucoma (PACG) admitted for mitomycin (MMC) augmented primary trabeculectomy due to failure in achieving target pressure after maximum medical therapy were selected. Age-matched non-glaucoma patients were controls. Impression cytology of the conjunctiva was obtained twice from glaucoma patients (1 week before augmented primary trabeculectomy and 3 months after surgery) and once from controls. Conjunctival cells were tagged with an anti-TGF-β antibody and analyzed by flow cytometry.

RESULTS: Eighteen patients (11 POAG and 7 PACG patients) and 18 age-matched control patients were included. Conjunctival TGF-β levels were significantly different between glaucoma (35.21% ± 14.12%) and control patients (14.96% ± 6.34%) (p = 0.001). There was a significant reduction in conjunctival TGF-β levels after augmented trabeculectomy (23.0% ± 13.8%) (p < 0.001). A significantly greater reduction in conjunctival TGF-β levels (61.6% ± 17.9%) was associated with complete success of trabeculectomy at 3 months (83.3%) after surgical intervention (p = 0.029).

CONCLUSION: The reduction of TGF-β on the conjunctival post primary augmented trabeculectomy may suggest TGF-β as potential predicting marker of short term trabeculectomy success. However, the result may be affected by site of impression, topical pressure lowering drugs and small sample size.

Keywords: Conjunctival TGFbeta; impression cytology; trabeculectomy

References

  1. Curr Drug Targets. 2006 May;7(5):547-65 - PubMed
  2. Am J Ophthalmol. 1968 Oct;66(4):673-9 - PubMed
  3. Surv Ophthalmol. 1987 Nov-Dec;32(3):149-70 - PubMed
  4. Exp Eye Res. 1994 Dec;59(6):723-7 - PubMed
  5. Surg Today. 1999;29(1):51-4 - PubMed
  6. Ophthalmology. 2007 Oct;114(10):1822-30 - PubMed
  7. Br J Ophthalmol. 1994 Jun;78(6):466-75 - PubMed
  8. Curr Eye Res. 1995 Mar;14(3):235-41 - PubMed
  9. Br J Ophthalmol. 1993 Sep;77(9):590-6 - PubMed
  10. Med J Malaysia. 2003 Oct;58(4):565-78 - PubMed
  11. Curr Eye Res. 1990 Oct;9(10):963-9 - PubMed
  12. Ophthalmology. 2002 Mar;109(3):427-31 - PubMed
  13. Curr Opin Ophthalmol. 2000 Apr;11(2):121-6 - PubMed
  14. Singapore Med J. 1996 Oct;37(5):505-7 - PubMed
  15. Invest Ophthalmol Vis Sci. 1999 Sep;40(10):2225-34 - PubMed
  16. Arch Ophthalmol. 1993 Feb;111(2):263-7 - PubMed
  17. Ophthalmology. 1995 Dec;102(12):1753-9 - PubMed
  18. Br J Ophthalmol. 1999 Jul;83(7):814-21 - PubMed
  19. J Clin Invest. 1989 May;83(5):1661-6 - PubMed
  20. Curr Eye Res. 2011 Apr;36(4):301-9 - PubMed
  21. Curr Eye Res. 1996 Mar;15(3):341-3 - PubMed
  22. Exp Eye Res. 2005 Jul;81(1):1-4 - PubMed
  23. Dis Markers. 2009;27(1):23-8 - PubMed
  24. Br J Ophthalmol. 2000 Jun;84(6):648-50 - PubMed
  25. Arch Ophthalmol. 1993 Jun;111(6):831-6 - PubMed

Publication Types