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Ohkura N, Yokouchi H, Mimura M, et al. Screening for hemostatic activities of popular Chinese medicinal herbs in vitro. J Intercult Ethnopharmacol. 2015;4(1):19-23doi: 10.5455/jice.20141128032845.
Ohkura, N., Yokouchi, H., Mimura, M., Nakamura, R., & Atsumi, G. (2015). Screening for hemostatic activities of popular Chinese medicinal herbs in vitro. Journal of intercultural ethnopharmacology, 4(1), 19-23. https://doi.org/10.5455/jice.20141128032845
Ohkura, Naoki, et al. "Screening for hemostatic activities of popular Chinese medicinal herbs in vitro." Journal of intercultural ethnopharmacology vol. 4,1 (2015): 19-23. doi: https://doi.org/10.5455/jice.20141128032845
Ohkura N, Yokouchi H, Mimura M, Nakamura R, Atsumi G. Screening for hemostatic activities of popular Chinese medicinal herbs in vitro. J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):19-23. doi: 10.5455/jice.20141128032845. Epub 2015 Jan 03. PMID: 26401379; PMCID: PMC4566763.
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J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):19-23. doi: 10.5455/jice.20141128032845. Epub 2015 Jan 03.

Screening for hemostatic activities of popular Chinese medicinal herbs in vitro.

Journal of intercultural ethnopharmacology

Naoki Ohkura, Haruna Yokouchi, Mariyo Mimura, Riki Nakamura, Gen-Ichi Atsumi

Affiliations

  1. Department of Molecular Physiology and Pathology, School of Pharma-Sciences, Teikyo University, Kaga, Itabashi, Tokyo, Japan.

PMID: 26401379 PMCID: PMC4566763 DOI: 10.5455/jice.20141128032845

Abstract

AIMS: This study aimed to identify new hemostyptics by assessing the coagulation enhancing activity of 114 Chinese herbal extracts in vitro.

METHODS: Herbs were boiled in water for 30 min, filtered and then lyophilized filtrates (10 mg/mL) were dissolved in water. Coagulation was assayed as prothrombin time (PT). Plasma diluted in saline was incubated with each extract for 5 min and then PT reagent was added, followed by CaCl2 solution and the time taken to form clots was measured. Extracts that decreased coagulation time were regarded as containing active compounds. The abilities of extracts to activate Factor XII were assessed and the activated form of factor XII (XIIa) was resolved by SDS-PAGE and visualized by silver staining.

RESULTS: Coagulation time was obviously shortened by extracts of Alpinia Rhizome, Areca, Artemisia Leaf, Cassia Bark, Danshen Root, Ephedra Herb, Epimedium Herb, Forsythia Fruit, Great Burdock Achene, Moutan Bark, Perilla Herb, Red Paeony Root, Schizonepeta Spike, Senticosus Rhizome, Sweet Annie, Uncaria Thorn and Zanthoxylum Peel. Factor XII was obviously activated by extracts of Artemisia Leaf and Great Burdock Achene, and slightly by Perilla herb.

CONCLUSION: Some popular Chinese medicinal herbs have potential as hemostatic agents and could thus be develope as new strategies for the treatment and prevention of bleeding.

Keywords: Hemostatic; herbal treatment; screening

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