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Rocha Mde F, Pine MB, Oliveira EF, et al. Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae). Comp Cytogenet. 2015;9(3):435-50doi: 10.3897/CompCytogen.v9i3.5160.
Rocha, M. d. e. . F., Pine, M. B., Oliveira, E. F., Loreto, V., Gallo, R. B., da Silva, C. R., de Domenico, F. C., & da Rosa, R. (2015). Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae). Comparative cytogenetics, 9(3), 435-50. https://doi.org/10.3897/CompCytogen.v9i3.5160
Rocha, Marília de França, et al. "Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae)." Comparative cytogenetics vol. 9,3 (2015): 435-50. doi: https://doi.org/10.3897/CompCytogen.v9i3.5160
Rocha Mde F, Pine MB, Oliveira EF, Loreto V, Gallo RB, da Silva CR, de Domenico FC, da Rosa R. Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae). Comp Cytogenet. 2015 Jul 24;9(3):435-50. doi: 10.3897/CompCytogen.v9i3.5160. eCollection 2015. PMID: 26312132; PMCID: PMC4547036.
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Comp Cytogenet. 2015 Jul 24;9(3):435-50. doi: 10.3897/CompCytogen.v9i3.5160. eCollection 2015.

Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae).

Comparative cytogenetics

Marília de França Rocha, Mariana Bozina Pine, Elizabeth Felipe Alves Dos Santos Oliveira, Vilma Loreto, Raquel Bozini Gallo, Carlos Roberto Maximiano da Silva, Fernando Campos de Domenico, Renata da Rosa

Affiliations

  1. Departamento de Biologia, ICB, Universidade de Pernambuco, Recife, Pernambuco, Brazil.
  2. Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
  3. Departamento de Genética, CCB, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  4. Museu de Zoologia, Instituto de Biociência, Universidade de São Paulo, São Paulo, São Paulo, Brazil.

PMID: 26312132 PMCID: PMC4547036 DOI: 10.3897/CompCytogen.v9i3.5160

Abstract

Tropidacris Scudder, 1869 is a genus widely distributed throughout the Neotropical region where speciation was probably promoted by forest reduction during the glacial and interglacial periods. There are no cytogenetic studies of Tropidacris, and information allowing inference or confirmation of the evolutionary events involved in speciation within the group is insufficient. In this paper, we used cytogenetic markers in two species, Tropidacriscollaris (Stoll, 1813) and Tropidacriscristatagrandis (Thunberg, 1824), collected in different Brazilian biomes. Both species exhibited 2n=24,XX for females and 2n=23,X0 for males. All chromosomes were acrocentric. There were some differences in the karyotype macrostructure, e.g. in the chromosome size. A wide interspecific variation in the chromosome banding (C-banding and CMA3/DAPI staining) indicated strong differences in the distribution of repetitive DNA sequences. Specifically, Tropidacriscristatagrandis had a higher number of bands in relation to Tropidacriscollaris. FISH with 18S rDNA revealed two markings coinciding with the NORs in both species. However, two analyzed samples of Tropidacriscollaris revealed a heterozygous condition for the rDNA site of S10 pair. In Tropidacriscollaris, the histone H3 genes were distributed on three chromosome pairs, whereas in Tropidacriscristatagrandis, these genes were observed on 14 autosomes and on the X chromosome, always in terminal regions. Our results demonstrate that, although the chromosome number and morphology are conserved in the genus, Tropidacriscristatagrandis substantially differs from Tropidacriscollaris in terms of the distribution of repetitive sequences. The devastation and fragmentation of the Brazilian rainforest may have led to isolation between these species, and the spreading of these repetitive sequences could contribute to speciation within the genus.

Keywords: 18S rDNA; Chromosome banding; histone H3 gene; repetitive DNA; speciation

References

  1. Experientia. 1980 Aug 15;36(8):1014-5 - PubMed
  2. Cytobios. 2000;103(403):111-9 - PubMed
  3. PLoS One. 2013 Jun 27;8(6):e66532 - PubMed
  4. New Phytol. 2004 Jan;161(1):107-112 - PubMed
  5. Mol Cytogenet. 2013 Oct 03;6(1):41 - PubMed
  6. Genetica. 2015 Feb;143(1):55-62 - PubMed
  7. Cell. 1989 Mar 10;56(5):771-6 - PubMed
  8. Cytogenet Genome Res. 2011;132(1-2):89-93 - PubMed
  9. Chromosome Res. 2009;17(3):397-404 - PubMed
  10. Genet Mol Biol. 2011 Apr;34(2):214-9 - PubMed
  11. EMBO J. 2002 Mar 1;21(5):1121-31 - PubMed
  12. Genetica. 2005 Nov;125(2-3):253-60 - PubMed
  13. Proc Natl Acad Sci U S A. 1986 May;83(9):2934-8 - PubMed
  14. Nat Rev Genet. 2010 Mar;11(3):175-80 - PubMed
  15. Mol Cytogenet. 2011 Nov 10;4:24 - PubMed
  16. Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12084-8 - PubMed
  17. Nat Rev Genet. 2007 Jan;8(1):35-46 - PubMed
  18. Chromosome Res. 2008;16(2):233-41 - PubMed
  19. Exp Cell Res. 1972 Nov;75(1):304-6 - PubMed
  20. Cytogenet Genome Res. 2013;141(4):309-16 - PubMed
  21. Chromosome Res. 2009;17(3):291-304 - PubMed
  22. Nat Cell Biol. 2007 Jan;9(1):19-20 - PubMed
  23. Trends Endocrinol Metab. 2000 May-Jun;11(4):142-8 - PubMed
  24. Genetica. 2011 Jul;139(7):921-31 - PubMed
  25. Trends Ecol Evol. 2001 Jul 1;16(7):351-358 - PubMed
  26. Genet Mol Biol. 2013 Sep;36(3):336-40 - PubMed

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