Indian J Clin Biochem. 2012 Jul;27(3):221-30. doi: 10.1007/s12291-012-0209-8. Epub 2012 May 08.

Analysis of Modification of Liver Proteome in Diabetic Rats by 2D Electrophoresis and MALDI-TOF-MS.

Indian journal of clinical biochemistry : IJCB

Dhanaraj Karthik, Soundherrajan Ilavenil, Balasubramanian Kaleeswaran, Sivanesan Ravikumar

PMID: 26405379 PMCID: PMC4577502 DOI: 10.1007/s12291-012-0209-8

Abstract

The uncontrolled hyperglycemia can lead to disturbances in the cell structure and functions of organs. This study was performed to analyze the "differential proteome" change in rat liver associated with diabetes mellitus in relation to effects of an anti-diabetic herb, Cynodon dactylon leaf extracts. Rats were intraperitoneally injected with alloxan (150 mg/kg/bw) and treated with C. dactylon leaf extracts (450 mg/kg/bw/day/orally). The liver proteins were subjected to proteome analysis using the advanced technologies i.e., 2D electrophoresis (2-DE) and mass spectrometry. Comparison of 2-DE protein distribution profiles among the livers from normal, alloxan-induced diabetic rats and alloxan-induced diabetic rats treated with C. dactylon leaves identified three proteins that were up-regulated in alloxan-induced diabetic rats i.e., nucleophosmin, l-xylulose reductase and carbonic anhydrase III which are known to be mainly involved in ribosome biogenesis, centrosome duplication, cell proliferation, tumor suppression, glucose metabolism, osmo-regulation, water-CO2 balance and acid-base balance. These results help us to understand the elucidation of molecular mechanism connected to liver function and insulin associated with diabetes mellitus. These identified proteins were primarily involved in cell proliferation and homoeostasis of liver tissues upon the treatment with C. dactylon leaf extracts.

Keywords: 2D Electrophoresis; Alloxan diabetes; MALDI-TOF-MS/MS; Proteomics; Rat liver

References

1. J Ethnopharmacol. 1999 Jun;65(3):181-202 - PubMed
2. Proteins. 1993 May;16(1):29-42 - PubMed
3. Protein Expr Purif. 2003 Mar;28(1):151-7 - PubMed
4. Proteomics. 2005 Mar;5(4):1003-12 - PubMed
5. Nature. 2005 Sep 1;437(7055):147-53 - PubMed
6. Curr Opin Chem Biol. 2000 Oct;4(5):489-94 - PubMed
7. J Mol Endocrinol. 2005 Apr;34(2):299-315 - PubMed
8. Cancer Detect Prev. 2006;30(6):481-90 - PubMed
9. Mem Inst Oswaldo Cruz. 2005 Mar;100 Suppl 1:127-30 - PubMed
10. Biosci Rep. 1988 Oct;8(5):401-6 - PubMed
11. Arch Biochem Biophys. 1981 Oct 15;211(2):632-42 - PubMed
12. J Ethnopharmacol. 2003 Feb;84(2-3):131-8 - PubMed
13. Ann N Y Acad Sci. 2005 Dec;1061:190-202 - PubMed
14. Structure. 2004 Dec;12(12):2149-60 - PubMed
15. J Ethnopharmacol. 2001 Jan;74(1):17-40 - PubMed
16. Physiol Res. 2001;50(6):537-46 - PubMed
17. Cancer Epidemiol Biomarkers Prev. 2007 Dec;16(12):2615-22 - PubMed
18. Free Radic Biol Med. 2008 Mar 15;44(6):1191-202 - PubMed
19. Physiol Rev. 1996 Oct;76(4):1109-61 - PubMed
20. J Biol Chem. 2002 May 17;277(20):17883-91 - PubMed
21. Chem Biol Interact. 2001 Jan 30;130-132(1-3):879-89 - PubMed
22. J Ethnopharmacol. 1999 Mar;64(3):195-210 - PubMed
23. Science. 2003 Apr 25;300(5619):633-6 - PubMed
24. Hum Reprod. 2003 May;18(5):915-24 - PubMed
25. Evid Based Complement Alternat Med. 2008 Dec;5(4):415-20 - PubMed
26. J Biol Chem. 1996 Jul 26;271(30):17718-23 - PubMed
27. Electrophoresis. 1999 Dec;20(18):3551-67 - PubMed
28. Hum Reprod. 2004 Jun;19(6):1438-47 - PubMed
29. J Ethnopharmacol. 2000 Jan;69(1):45-56 - PubMed
30. FEBS Lett. 1996 Oct 21;395(2-3):174-8 - PubMed
31. Anal Biochem. 1976 May 7;72:248-54 - PubMed
32. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
33. Eur J Med Res. 1997 Nov 28;2(11):457-64 - PubMed
34. Biomed Environ Sci. 2011 Apr;24(2):190-9 - PubMed
35. Dev Biol. 2002 Feb 15;242(2):236-54 - PubMed
36. Mol Cell Biol. 2008 Dec;28(23):7050-65 - PubMed
37. J Androl. 2001 Mar-Apr;22(2):302-15 - PubMed
38. Am J Respir Crit Care Med. 2000 Aug;162(2 Pt 1):707-15 - PubMed
39. J Cell Biochem. 2003 Dec 15;90(6):1140-8 - PubMed

Publication Types

• Journal Article