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Oehlke E, Lengkeek NA, So Le V, et al. The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)]. Appl Radiat Isot. 2015;107:13-16doi: 10.1016/j.apradiso.2015.09.008.
Oehlke, E., Lengkeek, N. A., So Le, V., Pellegrini, P. A., Greguric, I., & Weiner, R. (2016). The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)]. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 10713-16. https://doi.org/10.1016/j.apradiso.2015.09.008
Oehlke, Elisabeth, et al. "The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)]." Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine vol. 107 (2016): 13-16. doi: https://doi.org/10.1016/j.apradiso.2015.09.008
Oehlke E, Lengkeek NA, So Le V, Pellegrini PA, Greguric I, Weiner R. The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)]. Appl Radiat Isot. 2016 Jan;107:13-16. doi: 10.1016/j.apradiso.2015.09.008. Epub 2015 Sep 11. PMID: 26405839.
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Appl Radiat Isot. 2016 Jan;107:13-16. doi: 10.1016/j.apradiso.2015.09.008. Epub 2015 Sep 11.

The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)].

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

Elisabeth Oehlke, Nigel A Lengkeek, Van So Le, Paul A Pellegrini, Ivan Greguric, Ron Weiner

Affiliations

  1. Life Sciences Department, Building 1, Australian Nuclear Science and Technology Organisation (ANSTO), Locked bag 2001, Kirrawee DC, NSW 2232, Australia; Delft University of Technology, Mekelweg 15, 2629 JB Delft, The Netherlands. Electronic address: [email protected].
  2. Life Sciences Department, Building 1, Australian Nuclear Science and Technology Organisation (ANSTO), Locked bag 2001, Kirrawee DC, NSW 2232, Australia.

PMID: 26405839 DOI: 10.1016/j.apradiso.2015.09.008

Abstract

[(68)Ga(DOTATATE)] has demonstrated its clinical usefulness. Both Fe(3+) and Cu(2+), potential contaminants in Gallium-68 generator eluent, substantially reduce the radiochemical (RC) yield of [(68)Ga(DOTATATE)] if the metal/ligand ratio of 1:1 is exceeded. A variety of compounds were examined for their potential ability to reduce this effect. Most had no effect on RC yield. However, addition of phosphate diminished the influence of Fe(3+) by likely forming an insoluble iron salt. Addition of ascorbic acid reduced Cu(2+) and Fe(3+) to Cu(+) and Fe(2+) respectively, both of which have limited impact on RC yields. At low ligand amounts (5 nmol DOTATATE), the addition of 30 nmol phosphate (0.19 mM) increased the tolerance of Fe(3+) from 4 nmol to 10 nmol (0.06 mM), while the addition of ascorbic acid allowed high RC yields (>95%) in the presence of 40 nmol Fe(3+) (0.25 mM) and 100 nmol Cu(2+) (0.63 mM). The effect of ascorbic acid was highly pH-dependant, and gave optimal results at pH 3.

Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

Keywords: (68)Ga radiolabelling; Additives; Ascorbic acid; DOTATATE; Metal ion contamination; PET.; Phosphate

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