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Pinheiro GL, Correa RF, Cunha RS, et al. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Front Microbiol. 2015;6:860doi: 10.3389/fmicb.2015.00860.
Pinheiro, G. L., Correa, R. F., Cunha, R. S., Cardoso, A. M., Chaia, C., Clementino, M. M., Garcia, E. S., de Souza, W., & Frasés, S. (2015). Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Frontiers in microbiology, 6860. https://doi.org/10.3389/fmicb.2015.00860
Pinheiro, Guilherme L, et al. "Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica." Frontiers in microbiology vol. 6 (2015): 860. doi: https://doi.org/10.3389/fmicb.2015.00860
Pinheiro GL, Correa RF, Cunha RS, Cardoso AM, Chaia C, Clementino MM, Garcia ES, de Souza W, Frasés S. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Front Microbiol. 2015 Aug 20;6:860. doi: 10.3389/fmicb.2015.00860. eCollection 2015. PMID: 26347735; PMCID: PMC4542579.
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Front Microbiol. 2015 Aug 20;6:860. doi: 10.3389/fmicb.2015.00860. eCollection 2015.

Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica.

Frontiers in microbiology

Guilherme L Pinheiro, Raquel F Correa, Raquel S Cunha, Alexander M Cardoso, Catia Chaia, Maysa M Clementino, Eloi S Garcia, Wanderley de Souza, Susana Frasés

Affiliations

  1. Diretoria de Metrologia Aplicada às Ciências da Vida, Instituto Nacional de Metrologia, Qualidade e Tecnologia Rio de Janeiro, Brazil ; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil.
  2. Diretoria de Metrologia Aplicada às Ciências da Vida, Instituto Nacional de Metrologia, Qualidade e Tecnologia Rio de Janeiro, Brazil.
  3. Diretoria de Metrologia Aplicada às Ciências da Vida, Instituto Nacional de Metrologia, Qualidade e Tecnologia Rio de Janeiro, Brazil ; Centro Universitário Estadual da Zona Oeste, Unidade Universitária de Biologia Rio de Janeiro, Brazil.
  4. Departamento de Microbiologia, Instituto Nacional de Controle da Qualidade em Saúde, Fundação Oswaldo Cruz Rio de Janeiro, Brazil.
  5. Diretoria de Metrologia Aplicada às Ciências da Vida, Instituto Nacional de Metrologia, Qualidade e Tecnologia Rio de Janeiro, Brazil ; Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz Rio de Janeiro, Brazil.

PMID: 26347735 PMCID: PMC4542579 DOI: 10.3389/fmicb.2015.00860

Abstract

The enzymatic hydrolysis of cellulose by cellulases is one of the major limiting steps in the conversion of lignocellulosic biomass to yield bioethanol. To overcome this hindrance, significant efforts are underway to identify novel cellulases. The snail Achatina fulica is a gastropod with high cellulolytic activity, mainly due to the abundance of glycoside hydrolases produced by both the animal and its resident microbiota. In this study, we partially assessed the cellulolytic aerobic bacterial diversity inside the gastrointestinal tract of A. fulica by culture-dependent methods and evaluated the hydrolytic repertoire of the isolates. Forty bacterial isolates were recovered from distinct segments of the snail gut and identified to the genus level by 16S rRNA gene sequence analysis. Additional phenotypic characterization was performed using biochemical tests provided by the Vitek2 identification system. The overall enzymatic repertoire of the isolated strains was investigated by enzymatic plate assays, containing the following substrates: powdered sugarcane bagasse, carboxymethylcellulose (CMC), p-nitrophenyl-β-D-glucopyranoside (pNPG), p-nitrophenyl-β-D-cellobioside (pNPC), 4-methylumbelliferyl-β-D-glucopyranoside (MUG), 4-methylumbelliferyl-β-D-cellobioside (MUC), and 4-methylumbelliferyl-β-D-xylopyranoside (MUX). Our results indicate that the snail A. fulica is an attractive source of cultivable bacteria that showed to be valuable resources for the production of different types of biomass-degrading enzymes.

Keywords: Achatina fulica; carboxymethycellulose; cellulolytic bacteria; microbial diversity

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