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López-Castillo LM, López-Arciniega JA, Guerrero-Rangel A, et al. Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification. Front Plant Sci. 2015;6:670doi: 10.3389/fpls.2015.00670.
López-Castillo, L. M., López-Arciniega, J. A., Guerrero-Rangel, A., Valdés-Rodríguez, S., Brieba, L. G., García-Lara, S., & Winkler, R. (2015). Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification. Frontiers in plant science, 6670. https://doi.org/10.3389/fpls.2015.00670
López-Castillo, Laura M, et al. "Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification." Frontiers in plant science vol. 6 (2015): 670. doi: https://doi.org/10.3389/fpls.2015.00670
López-Castillo LM, López-Arciniega JA, Guerrero-Rangel A, Valdés-Rodríguez S, Brieba LG, García-Lara S, Winkler R. Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification. Front Plant Sci. 2015 Aug 31;6:670. doi: 10.3389/fpls.2015.00670. eCollection 2015. PMID: 26379694; PMCID: PMC4553411.
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Front Plant Sci. 2015 Aug 31;6:670. doi: 10.3389/fpls.2015.00670. eCollection 2015.

Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification.

Frontiers in plant science

Laura M López-Castillo, Janet A I López-Arciniega, Armando Guerrero-Rangel, Silvia Valdés-Rodríguez, Luis G Brieba, Silverio García-Lara, Robert Winkler

Affiliations

  1. Laboratory of Biochemical and Instrumental Analysis, Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato Irapuato, Mexico ; Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados - Instituto Politécnico Nacional Irapuato, Mexico.
  2. Laboratory of Biochemical and Instrumental Analysis, Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato Irapuato, Mexico.
  3. Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato Irapuato, Mexico.
  4. Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados - Instituto Politécnico Nacional Irapuato, Mexico.
  5. Plant-Food Molecular Breeding Unit, Tecnologico de Monterrey Monterrey, Mexico.

PMID: 26379694 PMCID: PMC4553411 DOI: 10.3389/fpls.2015.00670

Abstract

Plant peroxidases (PODs) are involved in diverse physiological processes, including defense against pathogens and insects. Contrary to their biological importance, only very few plant PODs have been proven on protein level, because their low abundance makes them difficult to detect in standard proteomics work-flows. A statistically significant positive correlation between POD activity and post-harvest insect resistance has been found for maize (Zea mays, p84C3) kernels. In combining activity-directed protein purification, genomic and proteomic tools we found that protein B6T173 (ZmPrx35) is responsible for the majority of the POD activity of the kernel. We successfully produced recombinant ZmPrx35 protein in Escherichia coli and demonstrate both, in vitro activity and the presence of a haem (heme) cofactor of the enzyme. Our findings support the screening for insect resistant maize variants and the construction of genetically optimized maize plants.

Keywords: activity-directed proteomics; insect resistance; low-abundance proteins; maize (Zea mays); peroxidase; plant proteomics

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