Display options
Cite
Emery SJ, Lacey E, Haynes PA. Data from a proteomic baseline study of Assemblage A in Giardia duodenalis. Data Brief. 2015;5:23-7doi: 10.1016/j.dib.2015.08.003.
Emery, S. J., Lacey, E., & Haynes, P. A. (2015). Data from a proteomic baseline study of Assemblage A in Giardia duodenalis. Data in brief, 523-7. https://doi.org/10.1016/j.dib.2015.08.003
Emery, Samantha J, et al. "Data from a proteomic baseline study of Assemblage A in Giardia duodenalis." Data in brief vol. 5 (2015): 23-7. doi: https://doi.org/10.1016/j.dib.2015.08.003
Emery SJ, Lacey E, Haynes PA. Data from a proteomic baseline study of Assemblage A in Giardia duodenalis. Data Brief. 2015 Aug 19;5:23-7. doi: 10.1016/j.dib.2015.08.003. eCollection 2015 Dec. PMID: 26380841; PMCID: PMC4556777.
Copy Download .nbib Format: NLM
Share it on

Data Brief. 2015 Aug 19;5:23-7. doi: 10.1016/j.dib.2015.08.003. eCollection 2015 Dec.

Data from a proteomic baseline study of Assemblage A in Giardia duodenalis.

Data in brief

Samantha J Emery, Ernest Lacey, Paul A Haynes

Affiliations

  1. Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
  2. Microbial Screening Technologies Pty Ltd, Smithfield, NSW 2165, Australia.

PMID: 26380841 PMCID: PMC4556777 DOI: 10.1016/j.dib.2015.08.003

Abstract

Eight Assemblage A strains from the protozoan parasite Giardia duodenalis were analysed using label-free quantitative shotgun proteomics, to evaluate inter- and intra-assemblage variation and complement available genetic and transcriptomic data. Isolates were grown in biological triplicate in axenic culture, and protein extracts were subjected to in-solution digest and online fractionation using Gas Phase Fractionation (GPF). Recent reclassification of genome databases for subassemblages was evaluated for database-dependent loss of information, and proteome composition of different isolates was analysed for biologically relevant assemblage-independent variation. The data from this study are related to the research article "Quantitative proteomics analysis of Giardia duodenalis Assemblage A - a baseline for host, assemblage and isolate variation" published in Proteomics (Emery et al., 2015 [1]).

Keywords: Assemblage A; Giardia duodenalis; Label-free quantitative shotgun proteomics; Parasite proteomics; Variable genome; Variant Surface Protein

References

  1. Anal Chem. 2008 Feb 15;80(4):1182-91 - PubMed
  2. Electrophoresis. 2011 Aug;32(16):2075-90 - PubMed
  3. Methods Mol Biol. 2013;1002:267-94 - PubMed
  4. Acta Trop. 1995 Sep;60(1):35-46 - PubMed
  5. Bioinformatics. 2004 Jun 12;20(9):1466-7 - PubMed
  6. Proteomics. 2015 Jul;15(13):2281-5 - PubMed
  7. Mol Biochem Parasitol. 1996 Feb-Mar;76(1-2):135-43 - PubMed
  8. Nucleic Acids Res. 2013 Jan;41(Database issue):D1063-9 - PubMed
  9. Trans R Soc Trop Med Hyg. 1983;77(4):487-8 - PubMed
  10. Methods Mol Biol. 2014;1072:289-302 - PubMed
  11. Int J Parasitol. 2000 Feb;30(2):129-36 - PubMed
  12. Anal Chem. 2003 Feb 15;75(4):768-74 - PubMed
  13. J Proteome Res. 2006 Sep;5(9):2339-47 - PubMed
  14. Int J Parasitol. 1989 Aug;19(5):519-27 - PubMed
  15. Parasitology. 1992 Jun;104 ( Pt 3):397-405 - PubMed
  16. Infect Immun. 2001 Oct;69(10):6503-10 - PubMed
  17. Anal Biochem. 1984 Apr;138(1):141-3 - PubMed

Publication Types