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Shabbir MZ, Jamil T, Ali AA, et al. Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan. Front Microbiol. 2015;6:917doi: 10.3389/fmicb.2015.00917.
Shabbir, M. Z., Jamil, T., Ali, A. A., Ahmad, A., Naeem, M., Chaudhary, M. H., Bilal, M., Ali, M. A., Muhammad, K., Yaqub, T., Bano, A., Mirza, A. I., Shabbir, M. A., McVey, W. R., Patel, K., Francesconi, S., Jayarao, B. M., & Rabbani, M. (2015). Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan. Frontiers in microbiology, 6917. https://doi.org/10.3389/fmicb.2015.00917
Shabbir, Muhammad Z, et al. "Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan." Frontiers in microbiology vol. 6 (2015): 917. doi: https://doi.org/10.3389/fmicb.2015.00917
Shabbir MZ, Jamil T, Ali AA, Ahmad A, Naeem M, Chaudhary MH, Bilal M, Ali MA, Muhammad K, Yaqub T, Bano A, Mirza AI, Shabbir MA, McVey WR, Patel K, Francesconi S, Jayarao BM, Rabbani M. Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan. Front Microbiol. 2015 Sep 10;6:917. doi: 10.3389/fmicb.2015.00917. eCollection 2015. PMID: 26441860; PMCID: PMC4564694.
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Front Microbiol. 2015 Sep 10;6:917. doi: 10.3389/fmicb.2015.00917. eCollection 2015.

Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan.

Frontiers in microbiology

Muhammad Z Shabbir, Tariq Jamil, Asad A Ali, Arfan Ahmad, Muhammad Naeem, Muhammad H Chaudhary, Muhammad Bilal, Muhammad A Ali, Khushi Muhammad, Tahir Yaqub, Asghari Bano, Ali I Mirza, Muhammad A B Shabbir, Walter R McVey, Ketan Patel, Stephen Francesconi, Bhushan M Jayarao, Masood Rabbani

Affiliations

  1. University of Veterinary and Animal Sciences Lahore, Pakistan.
  2. Quaid-i-Azam University Islamabad, Pakistan.
  3. University of the Punjab Lahore, Pakistan.
  4. Government College University Lahore, Pakistan.
  5. Department of Veterinary and Biomedical Sciences, The Pennsylvania State University University Park, PA, USA.
  6. Naval Medical Research Unit Frederick, MA, USA.

PMID: 26441860 PMCID: PMC4564694 DOI: 10.3389/fmicb.2015.00917

Abstract

A multidisciplinary, collaborative project was conducted to determine the prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan and ascertain its Public Health Significance. Using a grid-based sampling strategy, soil samples (n = 145) were collected from villages (n = 29, 5 samples/village) and examined for Bacillus anthracis, Burkholderia mallei/pseudomallei, Coxiella burnetii, Francisella tularensis, and Yersinia pestis using real time PCR assays. Chemical analysis of soil samples was also performed on these samples. The relationship between soil composition and absence or presence of the pathogen, and seven risk factors was evaluated. DNA of B. anthracis (CapB), B. mallei/pseudomallei (chromosomal gene), C. burnetii (IS1111, transposase gene), and F. tularensis (lipoprotein/outer membrane protein) was detected in 9.6, 1.4, 4.8, and 13.1% of soil samples, respectively. None of the samples were positive for protective antigen plasmid (PA) of B. anthracis and Y. pestis (plasminogen activating factor, pPla gene). The prevalence of B. anthracis (CapB) was found to be associated with organic matter, magnesium (Mg), copper (Cu), chromium (Cr), manganese (Mn), cobalt (Co), cadmium (Cd), sodium (Na), ferrous (Fe), calcium (Ca), and potassium (K). Phosphorous (P) was found to be associated with prevalence of F. tularensis while it were Mg, Co, Na, Fe, Ca, and K for C. burnetii. The odds of detecting DNA of F. tularensis were 2.7, 4.1, and 2.7 higher when soil sample sites were >1 km from animal markets, >500 m from vehicular traffic roads and animal density of < 1000 animals, respectively. While the odds of detecting DNA of C. burnetii was 32, 11.8, and 5.9 higher when soil sample sites were >500 m from vehicular traffic roads, presence of ground cover and animal density of < 1000 animals, respectively. In conclusion, the distribution pattern of the soil-borne pathogens in and around the areas of Lahore district puts both human and animal populations at a high risk of exposure. Further studies are needed to explore the genetic nature and molecular diversity of prevailing pathogens together with their seroconversion in animals and humans.

Keywords: Bacillus anthracis; Burkholderia mallei/pseudomallei; Coxiella burnetii; Francisella tularensis; Yersinia pestis; risk factors; soil chemistry

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