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Hatsuki R, Honda A, Kajitani M, et al. Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes. Front Microbiol. 2015;6:940doi: 10.3389/fmicb.2015.00940.
Hatsuki, R., Honda, A., Kajitani, M., & Yamamoto, T. (2015). Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes. Frontiers in microbiology, 6940. https://doi.org/10.3389/fmicb.2015.00940
Hatsuki, Ryuji, et al. "Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes." Frontiers in microbiology vol. 6 (2015): 940. doi: https://doi.org/10.3389/fmicb.2015.00940
Hatsuki R, Honda A, Kajitani M, Yamamoto T. Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes. Front Microbiol. 2015 Sep 09;6:940. doi: 10.3389/fmicb.2015.00940. eCollection 2015. PMID: 26441875; PMCID: PMC4563260.
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Front Microbiol. 2015 Sep 09;6:940. doi: 10.3389/fmicb.2015.00940. eCollection 2015.

Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes.

Frontiers in microbiology

Ryuji Hatsuki, Ayae Honda, Masayuki Kajitani, Takatoki Yamamoto

Affiliations

  1. Department of Mechanical and Control Engineering, Tokyo Institute of Technology Tokyo, Japan.
  2. Faculty of Bioscience and Applied Chemistry, Housei University Tokyo, Japan.
  3. Department of Bioscience, Teikyo University Tochigi, Japan.

PMID: 26441875 PMCID: PMC4563260 DOI: 10.3389/fmicb.2015.00940

Abstract

Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin. Currently, there is no methodology for continuously monitoring the environment for viruses in general, much less a methodology that allows the rapid and sensitive identification of a wide variety of viruses responsible for communicable diseases. Traditional approaches, based on PCR and immunodetection systems, only detect known or specifically targeted viruses. We here describe a simple device that can potentially detect any virus between nanogap electrodes using nonlinear impedance spectroscopy. Three test viruses, differing in shape and size, were used to demonstrate the general applicability of this approach: baculovirus, tobacco mosaic virus (TMV), and influenza virus. We show that each of the virus types responded differently in the nanogap to changes in the electric field strength, and the impedance of the virus solutions differed depending both on virus type and virus concentration. These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined. Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.

Keywords: environmental monitoring; impedance spectroscopy; nanofluidics; nanogap; virus; virus sensing

References

  1. Biophys J. 2011 Feb 2;100(3):637-45 - PubMed
  2. PLoS One. 2012;7(7):e42462 - PubMed
  3. Clin Microbiol Rev. 2006 Jan;19(1):165-256 - PubMed
  4. J Biol Eng. 2008 Apr 16;2:6 - PubMed
  5. Crit Rev Eukaryot Gene Expr. 2013;23(2):125-37 - PubMed
  6. Biosens Bioelectron. 2009 Dec 15;25(4):745-52 - PubMed
  7. Virology. 1982 Jul 30;120(2):433-40 - PubMed
  8. Biochim Biophys Acta. 2003 Jun 20;1622(1):57-63 - PubMed
  9. Biomed Microdevices. 2007 Dec;9(6):877-83 - PubMed
  10. Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14017-22 - PubMed
  11. J Vet Diagn Invest. 1993 Oct;5(4):510-5 - PubMed
  12. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1105-12 - PubMed
  13. Nat Methods. 2012 Jul;9(7):671-5 - PubMed
  14. J Fish Dis. 2011 Mar;34(3):189-202 - PubMed
  15. Talanta. 2009 Jul 15;79(2):159-64 - PubMed
  16. Lab Chip. 2008 Aug;8(8):1319-24 - PubMed
  17. Anal Chim Acta. 2008 Jul 14;620(1-2):8-26 - PubMed
  18. Comp Immunol Microbiol Infect Dis. 2009 Jul;32(4):341-50 - PubMed
  19. Eur Biophys J. 2001 Aug;30(4):268-72 - PubMed
  20. J Virol Methods. 2011 Dec;178(1-2):52-8 - PubMed
  21. Biosens Bioelectron. 2012 Oct-Dec;38(1):67-73 - PubMed
  22. Electrophoresis. 2014 Feb;35(2-3):433-40 - PubMed
  23. Biomicrofluidics. 2010 Jun 29;4(2):null - PubMed
  24. Biophys J. 1999 Jul;77(1):516-25 - PubMed
  25. Lancet Infect Dis. 2004 Jun;4(6):337-48 - PubMed
  26. Philos Trans R Soc Lond B Biol Sci. 1999 Mar 29;354(1383):531-5 - PubMed
  27. Anal Bioanal Chem. 2009 Jan;393(2):487-501 - PubMed
  28. Bioelectrochemistry. 2012 Dec;88:15-21 - PubMed

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