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Kou J, Takahashi S, Fan DY, et al. Partially dissecting the steady-state electron fluxes in Photosystem I in wild-type and pgr5 and ndh mutants of Arabidopsis. Front Plant Sci. 2015;6:758doi: 10.3389/fpls.2015.00758.
Kou, J., Takahashi, S., Fan, D. Y., Badger, M. R., & Chow, W. S. (2015). Partially dissecting the steady-state electron fluxes in Photosystem I in wild-type and pgr5 and ndh mutants of Arabidopsis. Frontiers in plant science, 6758. https://doi.org/10.3389/fpls.2015.00758
Kou, Jiancun, et al. "Partially dissecting the steady-state electron fluxes in Photosystem I in wild-type and pgr5 and ndh mutants of Arabidopsis." Frontiers in plant science vol. 6 (2015): 758. doi: https://doi.org/10.3389/fpls.2015.00758
Kou J, Takahashi S, Fan DY, Badger MR, Chow WS. Partially dissecting the steady-state electron fluxes in Photosystem I in wild-type and pgr5 and ndh mutants of Arabidopsis. Front Plant Sci. 2015 Sep 17;6:758. doi: 10.3389/fpls.2015.00758. eCollection 2015. PMID: 26442071; PMCID: PMC4584955.
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Front Plant Sci. 2015 Sep 17;6:758. doi: 10.3389/fpls.2015.00758. eCollection 2015.

Partially dissecting the steady-state electron fluxes in Photosystem I in wild-type and pgr5 and ndh mutants of Arabidopsis.

Frontiers in plant science

Jiancun Kou, Shunichi Takahashi, Da-Yong Fan, Murray R Badger, Wah S Chow

Affiliations

  1. College of Animal Science and Technology, Northwest A&F University Yangling, China ; Division of Plant Sciences, Research School of Biology, The Australian National University Canberra, ACT, Australia.
  2. Division of Plant Sciences, Research School of Biology, The Australian National University Canberra, ACT, Australia.
  3. Division of Plant Sciences, Research School of Biology, The Australian National University Canberra, ACT, Australia ; State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences Beijing, China.

PMID: 26442071 PMCID: PMC4584955 DOI: 10.3389/fpls.2015.00758

Abstract

Cyclic electron flux (CEF) around Photosystem I (PS I) is difficult to quantify. We obtained the linear electron flux (LEFO2) through both photosystems and the total electron flux through PS I (ETR1) in Arabidopsis in CO2-enriched air. ΔFlux = ETR1 - LEFO2 is an upper estimate of CEF, which consists of two components, an antimycin A-sensitive, PGR5 (proton gradient regulation 5 protein)-dependent component and an insensitive component facilitated by a chloroplastic nicotinamide adenine dinucleotide dehydrogenase-like complex (NDH). Using wild type as well as pgr5 and ndh mutants, we observed that (1) 40% of the absorbed light was partitioned to PS I; (2) at high irradiance a substantial antimycin A-sensitive CEF occurred in the wild type and the ndh mutant; (3) at low irradiance a sizable antimycin A-sensitive CEF occurred in the wild type but not in the ndh mutant, suggesting an enhancing effect of NDH in low light; and (4) in the pgr5 mutant, and the wild type and ndh mutant treated with antimycin A, a residual ΔFlux existed at high irradiance, attributable to charge recombination and/or pseudo-cyclic electron flow. Therefore, in low-light-acclimated plants exposed to high light, ΔFlux has contributions from various paths of electron flow through PS I.

Keywords: Arabidopsis; P700; antimycin A; cyclic electron flow; ndh mutant; pgr5 mutant; photosystem I

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