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Bogdanov IV, Finkina EI, Balandin SV, et al. Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein. Acta Naturae. 2015;7(3):65-73
Bogdanov, I. V., Finkina, E. I., Balandin, S. V., Melnikova, D. N., Stukacheva, E. A., & Ovchinnikova, T. V. (2015). Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein. Acta naturae, 7(3), 65-73.
Bogdanov, I V, et al. "Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein." Acta naturae vol. 7,3 (2015): 65-73.
Bogdanov IV, Finkina EI, Balandin SV, Melnikova DN, Stukacheva EA, Ovchinnikova TV. Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein. Acta Naturae. 2015 Jul-Sep;7(3):65-73. PMID: 26483961; PMCID: PMC4610166.
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Acta Naturae. 2015 Jul-Sep;7(3):65-73.

Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein.

Acta naturae

I V Bogdanov, E I Finkina, S V Balandin, D N Melnikova, E A Stukacheva, T V Ovchinnikova

Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow, Russia.

PMID: 26483961 PMCID: PMC4610166

Abstract

The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the α-helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major peach allergen Pru p 3. Both isoforms were shown to have immunological properties similar to those of other plant allergenic LTPs, but Lc-LTP3 displayed a less pronounced immunoreactivity.

Keywords: allergen; antimicrobial activity; cross-reactivity; heterologous expression; isoform; lentil; lipid binding; lipid transfer protein

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