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Geiger A, Tchicaya B, Rihet P. Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies. Genom Data. 2015;4:133-6doi: 10.1016/j.gdata.2015.04.007.
Geiger, A., Tchicaya, B., & Rihet, P. (2015). Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies. Genomics data, 4133-6. https://doi.org/10.1016/j.gdata.2015.04.007
Geiger, Anne, et al. "Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies." Genomics data vol. 4 (2015): 133-6. doi: https://doi.org/10.1016/j.gdata.2015.04.007
Geiger A, Tchicaya B, Rihet P. Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies. Genom Data. 2015 Apr 14;4:133-6. doi: 10.1016/j.gdata.2015.04.007. eCollection 2015 Jun. PMID: 26484198; PMCID: PMC4535939.
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Genom Data. 2015 Apr 14;4:133-6. doi: 10.1016/j.gdata.2015.04.007. eCollection 2015 Jun.

Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies.

Genomics data

Anne Geiger, Bernadette Tchicaya, Pascal Rihet

Affiliations

  1. UMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
  2. UMR1090 TAGC, INSERM, Marseille F-13288, France ; Aix-Marseille University, Marseille F-13288, France.

PMID: 26484198 PMCID: PMC4535939 DOI: 10.1016/j.gdata.2015.04.007

Abstract

Microarray is a powerful and cheap method to identify and quantify gene expression in particular in a mix of total RNA extracted from biological samples such as the tsetse fly gut, including several organisms (here, the fly tissue and the intestinal microorganisms). Besides, biostatistics and bioinformatics allow comparing the transcriptomes from samples collected from differently treated flies, and thus to identify and quantify differential expressed genes. Here, we describe in details a whole microarray transcriptome dataset produced from tsetse flies symbionts, Sodalis glossinidius and Wigglesworthia glossinidia. The tsetse fly midguts were sampled at key steps of tsetse fly infection by trypanosomes, 3-day and 10-day sampling times to target differentially expressed genes involved, respectively, in early events associated with trypanosome entry into the midgut and with the establishment of infection; 20 days to target the genes involved in events occurring later in the infection process. We describe in detail the methodology applied for analyzing the microarray data including differential expression as well as functional annotation of the identified symbiont genes. Both the microarray data and design are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48360;http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48361;http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55931.

Keywords: Genes differentially expressed; Glossina palpalis gambiensis; Microarrays; Sleeping sickness; Trypanosoma brucei gambiense; Tsetse fly infection

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