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Bonnardel J, Da Silva C, Masse M, et al. Gene expression profiling of the Peyer's patch mononuclear phagocyte system. Genom Data. 2015;5:21-4doi: 10.1016/j.gdata.2015.05.002.
Bonnardel, J., Da Silva, C., Masse, M., Montañana-Sanchis, F., Gorvel, J. P., & Lelouard, H. (2015). Gene expression profiling of the Peyer's patch mononuclear phagocyte system. Genomics data, 521-4. https://doi.org/10.1016/j.gdata.2015.05.002
Bonnardel, Johnny, et al. "Gene expression profiling of the Peyer's patch mononuclear phagocyte system." Genomics data vol. 5 (2015): 21-4. doi: https://doi.org/10.1016/j.gdata.2015.05.002
Bonnardel J, Da Silva C, Masse M, Montañana-Sanchis F, Gorvel JP, Lelouard H. Gene expression profiling of the Peyer's patch mononuclear phagocyte system. Genom Data. 2015 May 09;5:21-4. doi: 10.1016/j.gdata.2015.05.002. eCollection 2015 Sep. PMID: 26484215; PMCID: PMC4583621.
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Genom Data. 2015 May 09;5:21-4. doi: 10.1016/j.gdata.2015.05.002. eCollection 2015 Sep.

Gene expression profiling of the Peyer's patch mononuclear phagocyte system.

Genomics data

Johnny Bonnardel, Clément Da Silva, Marion Masse, Frederic Montañana-Sanchis, Jean-Pierre Gorvel, Hugues Lelouard

Affiliations

  1. Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université UM2, Inserm, U1104, CNRS UMR7280, 13288 Marseille, France.

PMID: 26484215 PMCID: PMC4583621 DOI: 10.1016/j.gdata.2015.05.002

Abstract

Peyer's patches (PPs) are primary inductive sites of mucosal immunity. The PP mononuclear phagocyte system, which encompasses both dendritic cells (DCs) and macrophages, is essential for the initiation of the mucosal immune response. We recently developed a method to isolate each mononuclear phagocyte subset of PP (Bonnardel et al., 2015). We performed a transcriptional analysis of three of these subsets: the CD11b(+) conventional DC, the lysozyme-expressing monocyte-derived DC termed LysoDC and the CD11c(hi) lysozyme-expressing macrophages. Here, we provide details of the gating strategy we used to isolate each phagocyte subset and show the quality controls and analysis associated with our gene array data deposited into Gene Expression Omnibus (GEO) under GSE65514.

Keywords: Dendritic cells; Macrophages; Mucosal immunity; Peyer's patches; Small intestine

References

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