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Ahn HY, Kim HH, Kim YA, et al. Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1. Endocrinol Metab (Seoul). 2015;30(4):584-92doi: 10.3803/EnM.2015.30.4.584.
Ahn, H. Y., Kim, H. H., Kim, Y. A., Kim, M., Ohn, J. H., Chung, S. S., Lee, Y. K., Park, D. J., Park, K. S., Moore, D. D., & Park, Y. J. (2015). Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1. Endocrinology and metabolism (Seoul, Korea), 30(4), 584-92. https://doi.org/10.3803/EnM.2015.30.4.584
Ahn, Hwa Young, et al. "Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1." Endocrinology and metabolism (Seoul, Korea) vol. 30,4 (2015): 584-92. doi: https://doi.org/10.3803/EnM.2015.30.4.584
Ahn HY, Kim HH, Kim YA, Kim M, Ohn JH, Chung SS, Lee YK, Park DJ, Park KS, Moore DD, Park YJ. Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1. Endocrinol Metab (Seoul). 2015 Dec;30(4):584-92. doi: 10.3803/EnM.2015.30.4.584. Epub 2015 Oct 20. PMID: 26485468; PMCID: PMC4722415.
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Endocrinol Metab (Seoul). 2015 Dec;30(4):584-92. doi: 10.3803/EnM.2015.30.4.584. Epub 2015 Oct 20.

Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1.

Endocrinology and metabolism (Seoul, Korea)

Hwa Young Ahn, Hwan Hee Kim, Ye An Kim, Min Kim, Jung Hun Ohn, Sung Soo Chung, Yoon Kwang Lee, Do Joon Park, Kyong Soo Park, David D Moore, Young Joo Park

Affiliations

  1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
  2. Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
  3. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
  4. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA. [email protected].
  5. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. [email protected].

PMID: 26485468 PMCID: PMC4722415 DOI: 10.3803/EnM.2015.30.4.584

Abstract

BACKGROUND: Expression of hepatic cholesterol 7α-hydroxylase (CYP7A1) is negatively regulated by orphan nuclear receptor small heterodimer partner (SHP). In this study, we aimed to find whether thyroid hormone regulates SHP expression by modulating the transcriptional activities of liver receptor homolog-1 (LRH-1).

METHODS: We injected thyroid hormone (triiodothyronine, T3) to C57BL/6J wild type. RNA was isolated from mouse liver and used for microarray analysis and quantitative real-time polymerase chain reaction (PCR). Human hepatoma cell and primary hepatocytes from mouse liver were used to confirm the effect of T3 in vitro. Promoter assay and electrophoretic mobility-shift assay (EMSA) were also performed using human hepatoma cell line.

RESULTS: Initial microarray results indicated that SHP expression is markedly decreased in livers of T3 treated mice. We confirmed that T3 repressed SHP expression in the liver of mice as well as in mouse primary hepatocytes and human hepatoma cells by real-time PCR analysis. LRH-1 increased the promoter activity of SHP; however, this increased activity was markedly decreased after thyroid hormone receptor β/retinoid X receptor α/T3 administration. EMSA revealed that T3 inhibits specific LRH-1 DNA binding.

CONCLUSION: We found that thyroid hormone regulates the expression of SHP mRNA through interference with the transcription factor, LRH-1.

Keywords: Bile acids and salts; Cholesterol; Liver receptor homolog-1; Small heterodimer partner; Thyroid hormones

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