Display options
Cite
Etemady A, Mohammdi M, Esmaelizad M, et al. Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran. Electron Physician. 2015;7(6):1381-6doi: 10.14661/1381.
Etemady, A., Mohammdi, M., Esmaelizad, M., Alamian, S., Vahedi, F., Aghaeipour, K., Behrozikhah, A. M., Faghihloo, E., Afshar, D., Firuzyar, S., & Rahimi, A. (2015). Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran. Electronic physician, 7(6), 1381-6. https://doi.org/10.14661/1381
Etemady, Afshar, et al. "Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran." Electronic physician vol. 7,6 (2015): 1381-6. doi: https://doi.org/10.14661/1381
Etemady A, Mohammdi M, Esmaelizad M, Alamian S, Vahedi F, Aghaeipour K, Behrozikhah AM, Faghihloo E, Afshar D, Firuzyar S, Rahimi A. Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran. Electron Physician. 2015 Oct 19;7(6):1381-6. doi: 10.14661/1381. eCollection 2015 Oct. PMID: 26516446; PMCID: PMC4623799.
Copy Download .nbib Format: NLM
Share it on

Electron Physician. 2015 Oct 19;7(6):1381-6. doi: 10.14661/1381. eCollection 2015 Oct.

Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran.

Electronic physician

Afshar Etemady, Mohsen Mohammdi, Majid Esmaelizad, Saeed Alamian, Fatemeh Vahedi, Khosro Aghaeipour, Ali Mohammad Behrozikhah, Ebrahim Faghihloo, Davoud Afshar, Sajad Firuzyar, Arian Rahimi

Affiliations

  1. Ph.D. of Medical Bacteriology, Department of Brucellosis, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  2. Assistant Professor, Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Lorestan University of Medical sciences, Khoramabad, Iran.
  3. Associate Professor, Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  4. Associate Professor, Department of Brucellosis, Razi Vaccine and Serum Research Institute, Mashhad, Iran.
  5. Ph.D. of Medical Virology, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  6. Ph.D. of Medical Bacteriology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  7. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  8. M.Sc. of Microbiology, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.

PMID: 26516446 PMCID: PMC4623799 DOI: 10.14661/1381

Abstract

INTRODUCTION: Brucella spp. are gram-negative, facultative intracellular bacteria pathogens responsible for brucellosis, a zoonotic disease that can cause abortion, fetal death, and genital infections in animals and undulant fever in humans. Lipopolysaccharide (LPS) is known as a major virulence factor of Brucella spp. The wboA gene is capable of encoding a glycosyltransferase that appears to play a major role in LPS biosynthesis. Hence, the characterization of this gene can help in the clarification of the pathogenicity of Brucella spp.

METHODS: This study was carried out at Razi Vaccine and Serum Research Institute in 2011. Briefly, the wboA gene in B. abortus biovar 3 and B. melitensis biovar 1, the predominant biovars in Iran, were amplified by using two pairs of specific primers. Polymerase chain reaction (PCR) products were cloned into a thymine-adenine (TA) cloning vector and transformed into an E. coli DH5α before being sequenced. Multiple alignments of identified sequences were performed, with all wboA sequences deposited in the GenBank sequence database.

RESULTS: This study showed that a mismatch has occurred in B. melitensis biovar 1; this biovar is predominant in Iran. In contrast, the wboA gene from B. abortus biovar 3 was similar to that of other B. abortus variations.

CONCLUSION: The comparison and alignment of the wboA gene of native Brucella strains in Iran to all wboA sequences deposited in GenBank revealed that the wboA gene has changed in the long term; hence, because of its unique nucleotide pattern, the gene can be used for specific diagnosis of B. abortus and B. canis.

Keywords: B. abortus; Brucella; LPS; wboA gene

References

  1. Vet Microbiol. 2002 Dec 20;90(1-4):147-55 - PubMed
  2. Vet Microbiol. 2010 Jan 27;140(3-4):392-8 - PubMed
  3. Vet Microbiol. 2002 Dec 20;90(1-4):479-96 - PubMed
  4. Rev Sci Tech. 2013 Apr;32(1):149-62 - PubMed
  5. BMC Infect Dis. 2013 Nov 01;13:514 - PubMed
  6. Infect Immun. 1984 Mar;43(3):779-82 - PubMed
  7. J Appl Microbiol. 2005;98(6):1270-81 - PubMed
  8. Infect Immun. 1998 Nov;66(11):5485-93 - PubMed
  9. BMC Microbiol. 2009 May 13;9:92 - PubMed
  10. Infect Immun. 1995 Aug;63(8):3054-61 - PubMed
  11. Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2688-93 - PubMed
  12. Clin Infect Dis. 2003 Oct 1;37(7):e95-9 - PubMed
  13. Microbiol Rev. 1993 Sep;57(3):655-82 - PubMed
  14. J Clin Microbiol. 1990 May;28(5):1057-9 - PubMed
  15. Clin Vaccine Immunol. 2013 Feb;20(2):174-80 - PubMed
  16. Infect Immun. 1981 Jan;31(1):362-70 - PubMed
  17. Infect Immun. 2000 Jul;68(7):3927-32 - PubMed
  18. Prev Vet Med. 2011 Nov 1;102(2):118-31 - PubMed
  19. J Clin Microbiol. 2004 Mar;42(3):1290-3 - PubMed
  20. Clin Diagn Lab Immunol. 1999 Sep;6(5):760-4 - PubMed
  21. J Bacteriol. 2005 Apr;187(8):2715-26 - PubMed
  22. Trends Biochem Sci. 2001 May;26(5):325-31 - PubMed
  23. Emerg Infect Dis. 1997 Apr-Jun;3(2):213-21 - PubMed

Publication Types