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Chang IY, Joester D. Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling. Microsc Microanal. 2015;21(6):1616-1621doi: 10.1017/S143192761501510X.
Chang, I. Y., & Joester, D. (2015). Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 21(6), 1616-1621. https://doi.org/10.1017/S143192761501510X
Chang, Irene Y T, and Joester, Derk. "Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling." Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada vol. 21,6 (2015): 1616-1621. doi: https://doi.org/10.1017/S143192761501510X
Chang IY, Joester D. Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling. Microsc Microanal. 2015 Dec;21(6):1616-1621. doi: 10.1017/S143192761501510X. Epub 2015 Oct 12. PMID: 26455924.
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Microsc Microanal. 2015 Dec;21(6):1616-1621. doi: 10.1017/S143192761501510X. Epub 2015 Oct 12.

Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

Irene Y T Chang, Derk Joester

Affiliations

  1. Department of Materials Science and Engineering,Northwestern University,2220 Campus Drive,Evanston,IL 60208,USA.

PMID: 26455924 DOI: 10.1017/S143192761501510X

Abstract

On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, "frozen-hydrated" specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (~700,000 µm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

Keywords: cryo-planning; cryoSEM; freeze fracture; high pressure freezing; ultrastructure

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