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Ross RL, Sauter AD, Limbach PA. Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides. J Mass Spectrom. 2015;50(10):1175-9doi: 10.1002/jms.3636.
Ross, R. L., Sauter, A. D., & Limbach, P. A. (2015). Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides. Journal of mass spectrometry : JMS, 50(10), 1175-9. https://doi.org/10.1002/jms.3636
Ross, Robert L, et al. "Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides." Journal of mass spectrometry : JMS vol. 50,10 (2015): 1175-9. doi: https://doi.org/10.1002/jms.3636
Ross RL, Sauter AD, Limbach PA. Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides. J Mass Spectrom. 2015 Oct;50(10):1175-9. doi: 10.1002/jms.3636. PMID: 26456787.
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J Mass Spectrom. 2015 Oct;50(10):1175-9. doi: 10.1002/jms.3636.

Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides.

Journal of mass spectrometry : JMS

Robert L Ross, Andrew D Sauter, Patrick A Limbach

Affiliations

  1. Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, PO Box 210172, Cincinnati, OH, 45221-0172, USA.
  2. Nanoliter LLC, Henderson, NV, USA.

PMID: 26456787 DOI: 10.1002/jms.3636

Abstract

Here, we report the utility of induction-based fluidics (IBF) for the introduction of oligonucleotides to a mass spectrometer via charged droplets. The device produces nanoliter-sized droplets, which are field transported with minimal concerns related to source plugging or sampling loss. The IBF source enabled detection of oligonucleotides at the nanomolar concentration level. Importantly, analysis of individual droplets revealed that oligonucleotide mixtures could be detected with ion abundance ratios that closely match the initial concentration ratios within the sample.

Copyright © 2015 John Wiley & Sons, Ltd.

Keywords: DNA; RNA; droplet mass spectrometry; ionization methods; nanospray

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