Display options
Cite
Lin L, Wang Y, Liu W, et al. BAMBI inhibits skin fibrosis in keloid through suppressing TGF-β1-induced hypernomic fibroblast cell proliferation and excessive accumulation of collagen I. Int J Clin Exp Med. 2015;8(8):13227-34
Lin, L., Wang, Y., Liu, W., & Huang, Y. (2015). BAMBI inhibits skin fibrosis in keloid through suppressing TGF-β1-induced hypernomic fibroblast cell proliferation and excessive accumulation of collagen I. International journal of clinical and experimental medicine, 8(8), 13227-34.
Lin, Lixin, et al. "BAMBI inhibits skin fibrosis in keloid through suppressing TGF-β1-induced hypernomic fibroblast cell proliferation and excessive accumulation of collagen I." International journal of clinical and experimental medicine vol. 8,8 (2015): 13227-34.
Lin L, Wang Y, Liu W, Huang Y. BAMBI inhibits skin fibrosis in keloid through suppressing TGF-β1-induced hypernomic fibroblast cell proliferation and excessive accumulation of collagen I. Int J Clin Exp Med. 2015 Aug 15;8(8):13227-34. eCollection 2015. PMID: 26550247; PMCID: PMC4612932.
Copy Download .nbib Format: NLM
Share it on

Int J Clin Exp Med. 2015 Aug 15;8(8):13227-34. eCollection 2015.

BAMBI inhibits skin fibrosis in keloid through suppressing TGF-β1-induced hypernomic fibroblast cell proliferation and excessive accumulation of collagen I.

International journal of clinical and experimental medicine

Lixin Lin, Yuting Wang, Wenyi Liu, Yong Huang

Affiliations

  1. Department of Plastic and Aesthetic Surgery, Yantai Yuhuangding Hospital Yantai City 264000, China.
  2. Department of Cardiac Surgery, Yantai Yuhuangding Hospital Yantai City 264000, China.

PMID: 26550247 PMCID: PMC4612932

Abstract

Keloids are scars characterized by pathologically excessive dermal fibrosis and aberrant wound healing. Hypernomic growth of fibroblast cells and excessive accumulation of collagens, especially collagen I, made important contribution to keloid formation. Transforming growth factor-beta 1 (TGF-β1) was a key ruler for the dermal fibrosis. Here, we found that BMP and activin membrane-bound inhibitor (BAMBI), a pseudo-receptor of TGF-β1, was being decreased during the human keloid development and in vitro keloid cell growth. To investigate the effect of BAMBI on keloid development, pcDNA-BAMBI expression vector were transfected into the human primary keloid cells. Then the cell proliferation and viability were detected with EdU and MTT methods and expression of TGF-β1 and collagen I/III were examined by Western blotting analysis. The results showed that, compared to the control, BAMBI overexpression suppressed the cell proliferation and expression of TGF-β1 and collagen I (P < 0.05), whereas, TGFβ1 overexpression rescued the suppression. Finally, pcDNA-BAMBI expression vector was subcutaneously injected into transplanted human keloid in nude mice. During the 6 weeks of in vivo experiment, pcDNA-BAMBI injection significantly suppressed the growth of the implanted keloids and the ratio of collagen I in the keloids. Therefore, BAMBI had an effect on inhibition of keloid growth through suppressing TGF-β1-induced fibroblast cell proliferation and excessive accumulation of collagen I.

Keywords: BMP and activin membrane-bound inhibitor; Keloid; collagens; fibroblast cell proliferation; transforming growth factor-beta 1

References

  1. Genomics. 2000 May 15;66(1):119-21 - PubMed
  2. Mol Med Rep. 2014 Aug;10(2):874-80 - PubMed
  3. Oncol Rep. 2013 Sep;30(3):1315-22 - PubMed
  4. Biochim Biophys Acta. 2013 Feb;1832(2):323-35 - PubMed
  5. Plast Reconstr Surg. 2010 Feb;125(2):557-68 - PubMed
  6. Am J Physiol Cell Physiol. 2013 Dec 1;305(11):C1098-113 - PubMed
  7. Tumour Biol. 2014 Jul;35(7):7047-56 - PubMed
  8. J Pediatr. 1993 Jan;122(1):1-9 - PubMed
  9. Arch Facial Plast Surg. 2006 Nov-Dec;8(6):362-8 - PubMed
  10. Dermatol Surg. 2009 Feb;35(2):171-81 - PubMed
  11. J Biol Chem. 2014 Mar 7;289(10):7082-91 - PubMed
  12. Curr Opin Genet Dev. 2006 Feb;16(1):30-7 - PubMed
  13. Cell Mol Life Sci. 2015 May;72(10):2023-39 - PubMed
  14. Int J Mol Sci. 2014 Sep 15;15(9):16257-69 - PubMed
  15. J Clin Invest. 2004 Aug;114(4):569-81 - PubMed
  16. Burns. 2014 Nov;40(7):1255-66 - PubMed
  17. Trans Am Clin Climatol Assoc. 2009;120:361-8 - PubMed

Publication Types