Display options
Cite
Nilsson O. Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs. Infect Ecol Epidemiol. 2015;5:28758doi: 10.3402/iee.v5.28758.
Nilsson, O. (2015). Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs. Infection ecology & epidemiology, 528758. https://doi.org/10.3402/iee.v5.28758
Nilsson, Oskar. "Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs." Infection ecology & epidemiology vol. 5 (2015): 28758. doi: https://doi.org/10.3402/iee.v5.28758
Nilsson O. Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs. Infect Ecol Epidemiol. 2015 Oct 20;5:28758. doi: 10.3402/iee.v5.28758. eCollection 2015. PMID: 26490763; PMCID: PMC4613903.
Copy Download .nbib Format: NLM
Share it on

Infect Ecol Epidemiol. 2015 Oct 20;5:28758. doi: 10.3402/iee.v5.28758. eCollection 2015.

Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs.

Infection ecology & epidemiology

Oskar Nilsson

Affiliations

  1. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, Sweden; [email protected].

PMID: 26490763 PMCID: PMC4613903 DOI: 10.3402/iee.v5.28758

Abstract

BACKGROUND: Raw food diets are popular among some dog owners, even though there are concerns regarding the infectious disease risk and public health implications. Hence, the two aims of this study were to investigate the hygiene quality of raw food diets for dogs in the Swedish market and if Escherichia coli with transferable resistance to extended spectrum cephalosporins (ESC) was present in such products.

METHODS: Samples of raw food diets were suspended and further diluted in 0.9% saline. Appropriate dilutions were 1) cultured on Petrifilm™SEC to quantify the amount of E. coli in the samples and 2) mixed with cefotaxime to a final concentration of 1 mg/L and cultured on Petrifilm™SEC to quantify the amount of ESC-resistant E. coli in the samples. Furthermore, undiluted suspensions were mixed 1:1 with double strength MacConkey broth with cefotaxime, enriched overnight and finally cultured on MacConkey agar with cefotaxime (1 mg/L). Suspected ESC-resistant E. coli were screened by PCR for genes encoding extended spectrum beta lactamases and plasmid-mediated AmpC and their susceptibility to a panel of antimicrobials was performed by broth microdilution using VetMIC GN-mo.

RESULTS: Escherichia coli was isolated from all samples (n=39) and ESC-resistant E. coli was isolated from nine samples (23%). All ESC-resistant E. coli were PCR-positive for the bla CMY-2 group and only one of them was also resistant to a non-beta-lactam antibiotic.

CONCLUSION: The results of this study indicate that raw food diets could be a source of ESC-resistant E. coli to dogs and highlight the need for maintaining good hygiene when handling these products to prevent infection.

Keywords: AmpC; BARF; E. coli; ESBL; raw food diet

References

  1. J Am Vet Med Assoc. 2006 Feb 15;228(4):537-42 - PubMed
  2. Can Vet J. 2011 Jan;52(1):50-4 - PubMed
  3. J Vet Diagn Invest. 1993 Jul;5(3):372-7 - PubMed
  4. Prev Vet Med. 2015 Apr 1;119(1-2):31-40 - PubMed
  5. Zoonoses Public Health. 2008 Oct;55(8-10):470-80 - PubMed
  6. Berl Munch Tierarztl Wochenschr. 2011 Mar-Apr;124(3-4):94-101 - PubMed
  7. Clin Microbiol Infect. 2013 Jul;19(7):E309-11 - PubMed
  8. J Am Vet Med Assoc. 2001 Nov 1;219(9):1222-5 - PubMed
  9. Zoonoses Public Health. 2008 Oct;55(8-10):462-9 - PubMed
  10. J Appl Microbiol. 2011 Feb;110(2):541-9 - PubMed
  11. Can Vet J. 2005 Jun;46(6):513-6 - PubMed
  12. Drug Resist Updat. 2013 Feb-Apr;16(1-2):22-45 - PubMed
  13. Zoonoses Public Health. 2012 Nov;59(7):513-20 - PubMed
  14. J Antimicrob Chemother. 2014 Jun;69(6):1497-500 - PubMed
  15. Vet Microbiol. 2014 Jul 16;171(3-4):315-20 - PubMed
  16. Can Vet J. 2002 Jun;43(6):441-2 - PubMed

Publication Types