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Krčková Z, Brouzdová J, Daněk M, et al. Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress. Front Plant Sci. 2015;6:928doi: 10.3389/fpls.2015.00928.
Krčková, Z., Brouzdová, J., Daněk, M., Kocourková, D., Rainteau, D., Ruelland, E., Valentová, O., Pejchar, P., & Martinec, J. (2015). Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress. Frontiers in plant science, 6928. https://doi.org/10.3389/fpls.2015.00928
Krčková, Zuzana, et al. "Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress." Frontiers in plant science vol. 6 (2015): 928. doi: https://doi.org/10.3389/fpls.2015.00928
Krčková Z, Brouzdová J, Daněk M, Kocourková D, Rainteau D, Ruelland E, Valentová O, Pejchar P, Martinec J. Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress. Front Plant Sci. 2015 Nov 04;6:928. doi: 10.3389/fpls.2015.00928. eCollection 2015. PMID: 26581502; PMCID: PMC4631941.
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Front Plant Sci. 2015 Nov 04;6:928. doi: 10.3389/fpls.2015.00928. eCollection 2015.

Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress.

Frontiers in plant science

Zuzana Krčková, Jitka Brouzdová, Michal Daněk, Daniela Kocourková, Dominique Rainteau, Eric Ruelland, Olga Valentová, Přemysl Pejchar, Jan Martinec

Affiliations

  1. Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague Prague, Czech Republic.
  2. Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
  3. 1ERL Inserm U1157/UMR7203, Faculté de Medecine Pierre et Marie Curie Paris, France.
  4. CNRS, UMR7618, Institut d'Ecologie et des Sciences de l'Environnement de Paris Créteil, France ; Université Paris Est, Institut d'Ecologie et des Sciences de l'Environnement de Paris, UPEC Créteil, France.
  5. Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague Prague, Czech Republic.

PMID: 26581502 PMCID: PMC4631941 DOI: 10.3389/fpls.2015.00928

Abstract

The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.

Keywords: Arabidopsis thaliana; diacylglycerol; heat stress; non-specific phospholipase C; phospholipids

References

  1. Front Plant Sci. 2015 Feb 16;6:66 - PubMed
  2. Plant Physiol. 1949 Jan;24(1):1-15 - PubMed
  3. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8569-74 - PubMed
  4. Prog Lipid Res. 2013 Jan;52(1):62-79 - PubMed
  5. Prog Lipid Res. 2012 Jul;51(3):208-20 - PubMed
  6. PLoS One. 2012;7(7):e41985 - PubMed
  7. Science. 2003 Aug 1;301(5633):653-7 - PubMed
  8. J Biol Chem. 2005 Mar 4;280(9):7469-76 - PubMed
  9. Plant Physiol. 2014 Apr;164(4):2184-96 - PubMed
  10. J Biosci Bioeng. 2007 Jul;104(1):34-41 - PubMed
  11. Plant J. 2008 Jan;53(2):264-74 - PubMed
  12. Methods Mol Biol. 2014;1209:1-11 - PubMed
  13. Biochem Biophys Res Commun. 2002 May 3;293(2):766-70 - PubMed
  14. New Phytol. 2009;182(1):175-87 - PubMed
  15. Cell. 2003 Jan 24;112(2):219-30 - PubMed
  16. Plant Sci. 2015 Feb;231:52-61 - PubMed
  17. Curr Biol. 2003 Aug 19;13(16):1378-87 - PubMed
  18. Plant J. 2009 Oct;60(1):10-21 - PubMed
  19. Plant Physiol. 2002 Feb;128(2):682-95 - PubMed
  20. Plant Signal Behav. 2015;10(6):e1031938 - PubMed
  21. Plant Cell Physiol. 2014 Nov;55(11):1873-83 - PubMed
  22. Plant J. 2009 Jul;59(1):169-78 - PubMed
  23. Plant Physiol. 2004 Jan;134(1):129-36 - PubMed
  24. Plant J. 2009 Dec;60(5):865-81 - PubMed
  25. Plant Cell. 2010 Aug;22(8):2642-59 - PubMed
  26. Plant Physiol. 2010 Aug;153(4):1895-906 - PubMed
  27. Protein Expr Purif. 1997 Aug;10(3):365-72 - PubMed
  28. Plant Signal Behav. 2012 Apr;7(4):472-5 - PubMed
  29. J Biol Chem. 2005 Jul 29;280(30):27578-86 - PubMed
  30. Biochem Biophys Res Commun. 2013 Mar 8;432(2):203-7 - PubMed
  31. Methods Mol Biol. 2012;913:251-61 - PubMed
  32. J Exp Bot. 2011 Jul;62(11):3753-63 - PubMed
  33. Plant Cell Environ. 2011 May;34(5):738-51 - PubMed
  34. Plant J. 2012 Feb;69(4):689-700 - PubMed
  35. Biochim Biophys Acta. 2010 Apr;1801(4):455-61 - PubMed
  36. FEBS Lett. 2006 Jul 24;580(17):4218-23 - PubMed
  37. Science. 2000 Jan 21;287(5452):476-9 - PubMed
  38. Plant J. 2011 Jun;66(5):844-51 - PubMed
  39. Sci Rep. 2015 May 27;5:10533 - PubMed
  40. Plant Signal Behav. 2010 Dec;5(12):1530-3 - PubMed
  41. Biochim Biophys Acta. 2002 Aug 19;1577(1):1-9 - PubMed
  42. Annu Rev Plant Biol. 2003;54:265-306 - PubMed
  43. Plant J. 2008 Oct;56(1):28-39 - PubMed
  44. J Microsc. 2004 May;214(Pt 2):159-73 - PubMed
  45. Plant Physiol. 1989 Jul;90(3):1134-42 - PubMed
  46. J Biol Chem. 2002 Aug 30;277(35):31994-2002 - PubMed
  47. Plant Cell. 2006 Mar;18(3):715-30 - PubMed
  48. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  49. Mol Plant. 2010 May;3(3):610-25 - PubMed
  50. New Phytol. 2010 Oct;188(1):150-60 - PubMed
  51. Methods Mol Biol. 2013;1009:193-203 - PubMed
  52. Plant J. 2004 Aug;39(4):527-36 - PubMed
  53. PLoS One. 2013 Apr 30;8(4):e62494 - PubMed
  54. Biosci Biotechnol Biochem. 2009 Dec;73(12):2632-9 - PubMed
  55. Plant Cell Environ. 2014 Sep;37(9):2002-13 - PubMed
  56. BMC Plant Biol. 2004 Sep 17;4:17 - PubMed
  57. Plant J. 2001 Jul;27(1):25-35 - PubMed
  58. Plant J. 2004 May;38(3):432-47 - PubMed

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