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Mance D, Sinnige T, Kaplan M, et al. An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy. Angew Chem Int Ed Engl. 2015;54(52):15799-803doi: 10.1002/anie.201509170.
Mance, D., Sinnige, T., Kaplan, M., Narasimhan, S., Daniëls, M., Houben, K., Baldus, M., & Weingarth, M. (2015). An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy. Angewandte Chemie (International ed. in English), 54(52), 15799-803. https://doi.org/10.1002/anie.201509170
Mance, Deni, et al. "An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy." Angewandte Chemie (International ed. in English) vol. 54,52 (2015): 15799-803. doi: https://doi.org/10.1002/anie.201509170
Mance D, Sinnige T, Kaplan M, Narasimhan S, Daniëls M, Houben K, Baldus M, Weingarth M. An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy. Angew Chem Int Ed Engl. 2015 Dec 21;54(52):15799-803. doi: 10.1002/anie.201509170. Epub 2015 Nov 11. PMID: 26555653; PMCID: PMC4691318.
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Angew Chem Int Ed Engl. 2015 Dec 21;54(52):15799-803. doi: 10.1002/anie.201509170. Epub 2015 Nov 11.

An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.

Angewandte Chemie (International ed. in English)

Deni Mance, Tessa Sinnige, Mohammed Kaplan, Siddarth Narasimhan, Mark Daniëls, Klaartje Houben, Marc Baldus, Markus Weingarth

Affiliations

  1. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands).
  2. Present address: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW (UK).
  3. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands). [email protected].
  4. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands). [email protected].

PMID: 26555653 PMCID: PMC4691318 DOI: 10.1002/anie.201509170

Abstract

(1) H-detection can greatly improve spectral sensitivity in biological solid-state NMR (ssNMR), thus allowing the study of larger and more complex proteins. However, the general requirement to perdeuterate proteins critically curtails the potential of (1) H-detection by the loss of aliphatic side-chain protons, which are important probes for protein structure and function. Introduced herein is a labelling scheme for (1) H-detected ssNMR, and it gives high quality spectra for both side-chain and backbone protons, and allows quantitative assignments and aids in probing interresidual contacts. Excellent (1) H resolution in membrane proteins is obtained, the topology and dynamics of an ion channel were studied. This labelling scheme will open new avenues for the study of challenging proteins by ssNMR.

© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Keywords: ion channels; membrane proteins; proton detection; side-chain protons; solid-state NMR spectroscopy

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