Display options
Cite
Moses C, Wheeler LP, LeVaillant CJ, et al. The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells. eNeuro. 2015;2(3)doi: 10.1523/ENEURO.0044-14.2015.
Moses, C., Wheeler, L. P., LeVaillant, C. J., Kramer, A., Ryan, M., Cozens, G. S., Sharma, A., Pollett, M. A., Rodger, J., & Harvey, A. R. (2015). The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells. eNeuro, 2(3), . https://doi.org/10.1523/ENEURO.0044-14.2015
Moses, Colette, et al. "The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells." eNeuro vol. 2,3 (2015). doi: https://doi.org/10.1523/ENEURO.0044-14.2015
Moses C, Wheeler LP, LeVaillant CJ, Kramer A, Ryan M, Cozens GS, Sharma A, Pollett MA, Rodger J, Harvey AR. The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells. eNeuro. 2015 Jul 10;2(3). doi: 10.1523/ENEURO.0044-14.2015. eCollection 2015. PMID: 26464991; PMCID: PMC4586937.
Copy Download .nbib Format: NLM
Share it on

eNeuro. 2015 Jul 10;2(3). doi: 10.1523/ENEURO.0044-14.2015. eCollection 2015.

The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells.

eNeuro

Colette Moses, Lachlan P G Wheeler, Chrisna J LeVaillant, Anne Kramer, Marisa Ryan, Greg S Cozens, Anil Sharma, Margaret A Pollett, Jennifer Rodger, Alan R Harvey

Affiliations

  1. School of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
  2. School of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Department of Neuroscience, Karolinska Institutet , 17177 Stockholm, Sweden.
  3. School of Animal Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Western Australian Neuroscience Research Institute , Nedlands, Western Australia 6009, Australia.
  4. School of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Western Australian Neuroscience Research Institute , Nedlands, Western Australia 6009, Australia.

PMID: 26464991 PMCID: PMC4586937 DOI: 10.1523/ENEURO.0044-14.2015

Abstract

Similar to neurons in the peripheral nervous system, immature CNS-derived RGCs become dependent on target-derived neurotrophic support as their axons reach termination sites in the brain. To study the factors that influence this developmental transition we took advantage of the fact that rat RGCs are born, and target innervation occurs, over a protracted period of time. Early-born RGCs have axons in the SC by birth (P0), whereas axons from late-born RGCs do not innervate the SC until P4-P5. Birth dating RGCs using EdU allowed us to identify RGCs (1) with axons still growing toward targets, (2) transitioning to target dependence, and (3) entirely dependent on target-derived support. Using laser-capture microdissection we isolated ∼34,000 EdU(+) RGCs and analyzed transcript expression by custom qPCR array. Statistical analyses revealed a difference in gene expression profiles in actively growing RGCs compared with target-dependent RGCs, as well as in transitional versus target-dependent RGCs. Prior to innervation RGCs expressed high levels of BDNF and CNTFR α but lower levels of neurexin 1 mRNA. Analysis also revealed greater expression of transcripts for signaling molecules such as MAPK, Akt, CREB, and STAT. In a supporting in vitro study, purified birth-dated P1 RGCs were cultured for 24-48 h with or without BDNF; lack of BDNF resulted in significant loss of early-born but not late-born RGCs. In summary, we identified several important changes in RGC signaling that may form the basis for the switch from target independence to dependence.

Keywords: BDNF; neurotrophic factors; programmed cell death; retinal ganglion cells; target dependence

References

  1. PLoS One. 2012;7(11):e49830 - PubMed
  2. Cell Rep. 2014 Aug 21;8(4):1006-17 - PubMed
  3. Methods. 2010 Apr;50(4):237-43 - PubMed
  4. Neuron. 2002 Aug 15;35(4):605-23 - PubMed
  5. J Neurosci. 2003 Nov 5;23 (31):10137-45 - PubMed
  6. Nature. 2008 Jan 24;451(7177):470-4 - PubMed
  7. Anat Rec (Hoboken). 2009 Dec;292(12):1976-85 - PubMed
  8. Exp Eye Res. 2006 Dec;83(6):1482-92 - PubMed
  9. Invest Ophthalmol Vis Sci. 2004 Aug;45(8):2737-46 - PubMed
  10. Science. 2004 Feb 13;303(5660):1026-30 - PubMed
  11. Nat Neurosci. 2007 Nov;10(11):1449-57 - PubMed
  12. Mol Cell Neurosci. 2010 May;44(1):43-54 - PubMed
  13. J Neurosci. 2004 Oct 6;24(40):8726-40 - PubMed
  14. Philos Trans R Soc Lond B Biol Sci. 2006 Sep 29;361(1473):1575-92 - PubMed
  15. Curr Opin Neurobiol. 1999 Feb;9(1):65-73 - PubMed
  16. Trends Neurosci. 1999 Dec;22(12):529-34 - PubMed
  17. J Neurobiol. 2001 Nov 15;49(3):173-87 - PubMed
  18. J Comp Neurol. 1983 Sep 20;219(3):356-68 - PubMed
  19. Neuron. 1999 May;23(1):139-48 - PubMed
  20. Brain Res Dev Brain Res. 1998 Apr 17;107(1):133-42 - PubMed
  21. Proc Natl Acad Sci U S A. 2015 May 5;112(18):5821-6 - PubMed
  22. Cytokine Growth Factor Rev. 2008 Jun-Aug;19(3-4):245-51 - PubMed
  23. Mol Cell Neurosci. 2004 Aug;26(4):544-57 - PubMed
  24. Nat Neurosci. 2004 Mar;7(3):221-8 - PubMed
  25. J Neurosci. 2004 Mar 3;24(9):2277-85 - PubMed
  26. J Neurosci. 2007 Aug 8;27(32):8593-603 - PubMed
  27. J Neurosci Res. 1998 Oct 1;54(1):27-37 - PubMed
  28. J Comp Neurol. 2007 Jun 20;502(6):1047-65 - PubMed
  29. Physiol Rev. 1980 Oct;60(4):1284-335 - PubMed
  30. J Int Med Res. 2008 Jul-Aug;36(4):815-29 - PubMed
  31. J Neurosci. 2003 Dec 17;23(37):11770-7 - PubMed
  32. J Neurosci. 2008 Aug 13;28(33):8150-60 - PubMed
  33. J Neurosci. 2006 May 17;26(20):5565-73 - PubMed
  34. J Neurobiol. 1994 Dec;25(12):1570-8 - PubMed
  35. Neuroscience. 1992;46(2):419-29 - PubMed
  36. Methods. 2001 Dec;25(4):402-8 - PubMed
  37. J Neurosci. 1984 Mar;4(3):767-74 - PubMed
  38. Gene Ther. 2012 Feb;19(2):127-36 - PubMed
  39. Cell Tissue Res. 2012 Jul;349(1):79-85 - PubMed
  40. Exp Neurol. 2008 Feb;209(2):321-32 - PubMed
  41. Gen Comp Endocrinol. 2008 May 1;156(3):613-21 - PubMed
  42. Neuron. 2002 Aug 15;35(4):711-9 - PubMed
  43. Nat Rev Neurosci. 2013 Jan;14(1):7-23 - PubMed
  44. Mol Cell Neurosci. 2009 Jul;41(3):313-24 - PubMed
  45. Neuron. 2000 Mar;25(3):563-74 - PubMed
  46. J Neurobiol. 2004 Sep 5;60(3):319-27 - PubMed
  47. Int J Physiol Pathophysiol Pharmacol. 2012;4(4):188-200 - PubMed
  48. J Neurosci. 1995 Dec;15(12):8143-55 - PubMed
  49. J Neurosci. 2010 Feb 3;30(5):1739-49 - PubMed
  50. Brain Res. 1995 Dec 15;704(1):121-4 - PubMed
  51. J Neurosci. 1995 Apr;15(4):3128-37 - PubMed
  52. J Neurosci. 2002 Sep 1;22(17):7639-49 - PubMed
  53. J Neurobiol. 2004 Apr;59(1):162-80 - PubMed
  54. Brain Res. 2010 Mar 10;1319:21-32 - PubMed
  55. Curr Opin Neurobiol. 2000 Jun;10(3):381-91 - PubMed
  56. Eur J Neurosci. 2004 Aug;20(3):843-7 - PubMed
  57. Philos Trans R Soc Lond B Biol Sci. 2006 Sep 29;361(1473):1545-64 - PubMed
  58. J Comp Neurol. 1986 Sep 8;251(2):240-59 - PubMed
  59. Nat Protoc. 2008;3(6):1101-8 - PubMed
  60. Invest Ophthalmol Vis Sci. 2003 Dec;44(12):5342-8 - PubMed
  61. Dev Neurobiol. 2010 Apr;70(5):350-9 - PubMed
  62. Annu Rev Neurosci. 2003;26:299-330 - PubMed
  63. J Neurosci. 2000 Jun 1;20(11):4081-90 - PubMed
  64. Neuropharmacology. 2014 Jan;76 Pt C:709-18 - PubMed
  65. EMBO Rep. 2008 Oct;9(10):1041-7 - PubMed
  66. EMBO J. 2005 Mar 9;24(5):1068-78 - PubMed
  67. Development. 1999 Oct;126(19):4365-73 - PubMed
  68. J Neurobiol. 1994 Nov;25(11):1454-66 - PubMed
  69. Front Mol Neurosci. 2012 Jan 12;4:60 - PubMed
  70. Neuron. 1995 Jan;14(1):117-24 - PubMed
  71. Cell Signal. 2006 Nov;18(11):1834-43 - PubMed
  72. Prog Neurobiol. 2002 Jun;67(3):203-33 - PubMed
  73. Vision Res. 2002 Jan;42(2):151-7 - PubMed
  74. Neurosci Lett. 2002 Jun 14;325(3):207-10 - PubMed
  75. Histol Histopathol. 1998 Apr;13(2):437-59 - PubMed
  76. J Neurosci. 1998 Mar 15;18(6):2097-107 - PubMed
  77. Brain. 2006 Jun;129(Pt 6):1517-33 - PubMed
  78. Exp Neurol. 2010 Oct;225(2):353-65 - PubMed
  79. Brain Res. 2006 Sep 19;1110(1):81-94 - PubMed
  80. Neuron. 2009 Nov 25;64(4):484-97 - PubMed
  81. J Neurosci. 2000 Jan 15;20(2):2-8 - PubMed
  82. J Neurosci. 2010 Apr 21;30(16):5635-43 - PubMed
  83. Neuron. 1998 Apr;20(4):709-26 - PubMed
  84. J Neurosci. 2008 Nov 26;28(48):12700-12 - PubMed
  85. Neural Dev. 2010 Nov 02;5:30 - PubMed
  86. Curr Biol. 2005 Apr 26;15(8):R302-5 - PubMed
  87. Cell. 2003 May 30;113(5):657-70 - PubMed
  88. Nature. 1987 Mar 26-Apr 1;326(6111):353-8 - PubMed
  89. Nat Neurosci. 2009 Nov;12(11):1407-14 - PubMed
  90. Neuron. 2002 Feb 28;33(5):689-702 - PubMed
  91. Neuroscience. 2005;131(2):387-95 - PubMed
  92. Neuroreport. 1994 Oct 3;5(15):1882-4 - PubMed
  93. J Neurosci. 2002 Dec 1;22(23 ):10324-32 - PubMed
  94. Invest Ophthalmol Vis Sci. 2008 Mar;49(3):975-85 - PubMed
  95. Dev Biol. 1989 Dec;136(2):296-303 - PubMed
  96. Science. 2001 Nov 30;294(5548):1945-8 - PubMed
  97. J Comp Neurol. 1992 Nov 1;325(1):83-94 - PubMed
  98. Prog Retin Eye Res. 2003 Jul;22(4):483-543 - PubMed
  99. Neurosci Lett. 1983 Apr 29;36(3):255-60 - PubMed
  100. Nucleic Acids Res. 2001 May 1;29(9):e45 - PubMed
  101. Mol Cell Neurosci. 1999 Jul;14(1):52-65 - PubMed
  102. Neuron. 1991 Nov;7(5):819-30 - PubMed
  103. PLoS One. 2012;7(2):e31105 - PubMed
  104. J Neurochem. 2002 Jan;80(2):239-54 - PubMed
  105. Dev Biol. 2007 Aug 1;308(1):1-14 - PubMed
  106. Mol Cell Neurosci. 2011 Feb;46(2):507-15 - PubMed
  107. Philos Trans R Soc Lond B Biol Sci. 1996 Mar 29;351(1338):389-94 - PubMed
  108. J Neurobiol. 2003 Aug;56(2):171-7 - PubMed
  109. Nature. 1989 Feb 9;337(6207):553-5 - PubMed
  110. Annu Rev Neurosci. 1991;14:453-501 - PubMed
  111. J Comp Neurol. 1976 Jan 15;165(2):247-64 - PubMed
  112. Brain Res. 1985 Mar;351(1):129-34 - PubMed
  113. Dev Neurobiol. 2010 Apr;70(5):271-88 - PubMed
  114. Nat Rev Mol Cell Biol. 2001 Aug;2(8):599-609 - PubMed
  115. Brain. 2008 Jan;131(Pt 1):250-63 - PubMed
  116. Brain Res. 1983 Jan;282(2):149-68 - PubMed
  117. Histol Histopathol. 2010 Feb;25(2):237-58 - PubMed
  118. Dev Biol. 1996 Oct 10;179(1):102-15 - PubMed
  119. Ups J Med Sci. 2010 Feb;115(1):65-80 - PubMed
  120. Cell. 2007 Jun 29;129(7):1261-74 - PubMed
  121. Biotechniques. 2007 Apr;42(4):503-12 - PubMed
  122. Dev Biol. 2007 Dec 15;312(2):596-612 - PubMed
  123. Curr Opin Neurobiol. 2001 Jun;11(3):297-305 - PubMed
  124. Neuroscience. 1981;6(5):931-44 - PubMed
  125. Nature. 2010 Sep 2;467(7311):59-63 - PubMed
  126. J Neurosci. 2004 Dec 1;24(48):10806-15 - PubMed
  127. Cell Mol Life Sci. 2014 Nov;71(22):4443-56 - PubMed
  128. Nature. 2004 Oct 14;431(7010):847-53 - PubMed
  129. Mol Cell Biol. 1999 Jan;19(1):751-63 - PubMed
  130. J Neurosci. 2007 Mar 7;27(10):2444-56 - PubMed
  131. J Mol Neurosci. 2005;26(1):17-25 - PubMed
  132. Mol Cell Neurosci. 2011 Jun;47(2):131-6 - PubMed
  133. Dev Neurobiol. 2011 Dec;71(12):1186-211 - PubMed
  134. Neuron. 1995 Oct;15(4):805-19 - PubMed
  135. Mol Cell Neurosci. 1997;9(5-6):397-408 - PubMed
  136. Eur J Neurosci. 2002 Oct;16(7):1295-304 - PubMed
  137. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3860-8 - PubMed
  138. Science. 2008 Nov 7;322(5903):963-6 - PubMed
  139. J Biol Chem. 2006 Jun 9;281(23):15636-44 - PubMed
  140. Nature. 1995 Nov 9;378(6553):192-6 - PubMed
  141. Int Rev Neurobiol. 2012;106:1-33 - PubMed
  142. Neuron. 1999 Apr;22(4):667-76 - PubMed
  143. Neuron. 1992 Jul;9(1):139-50 - PubMed
  144. Vis Neurosci. 1995 Mar-Apr;12(2):215-22 - PubMed
  145. Development. 2005 Oct;132(19):4285-98 - PubMed
  146. Prog Retin Eye Res. 2000 Sep;19(5):559-75 - PubMed
  147. J Neurosci. 1999 Nov 15;19(22):9716-27 - PubMed
  148. Science. 2003 Oct 31;302(5646):885-9 - PubMed
  149. Exp Neurol. 2001 Nov;172(1):81-91 - PubMed
  150. Mol Cell Neurosci. 2007 Mar;34(3):453-67 - PubMed
  151. J Neurobiol. 2004 May;59(2):216-35 - PubMed
  152. Exp Cell Res. 1999 Nov 25;253(1):255-70 - PubMed
  153. J Neurosci. 1986 Oct;6(10):3031-8 - PubMed
  154. Neuron. 2000 Aug;27(2):265-77 - PubMed
  155. Science. 2009 Oct 9;326(5950):298-301 - PubMed
  156. Neuron. 2005 Feb 3;45(3):345-51 - PubMed
  157. Curr Opin Neurobiol. 2004 Feb;14 (1):118-24 - PubMed
  158. Mol Cell Neurosci. 2006 May-Jun;32(1-2):143-54 - PubMed
  159. J Comp Neurol. 2004 Jun 21;474(2):304-24 - PubMed
  160. Proc Natl Acad Sci U S A. 1979 Jan;76(1):514-7 - PubMed
  161. Neuron. 2009 Mar 26;61(6):852-64 - PubMed
  162. J Neurosci. 2008 Oct 22;28(43):11015-23 - PubMed
  163. PLoS One. 2013;8(1):e54462 - PubMed
  164. Curr Opin Neurobiol. 2010 Aug;20(4):510-8 - PubMed
  165. Physiol Rev. 1968 Jul;48(3):534-69 - PubMed
  166. Cancer Res. 1999 Apr 1;59(7 Suppl):1693s-1700s - PubMed
  167. Exp Eye Res. 2009 Apr;88(4):808-15 - PubMed

Publication Types