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Woolley CF, Hayes MA. Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay. Anal Methods. 2015;7(20):8632-8639doi: 10.1039/C5AY01071C.
Woolley, C. F., & Hayes, M. A. (2015). Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay. Analytical methods : advancing methods and applications, 7(20), 8632-8639. https://doi.org/10.1039/C5AY01071C
Woolley, Christine F, and Hayes, Mark A. "Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay." Analytical methods : advancing methods and applications vol. 7,20 (2015): 8632-8639. doi: https://doi.org/10.1039/C5AY01071C
Woolley CF, Hayes MA. Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay. Anal Methods. 2015 Oct 21;7(20):8632-8639. doi: 10.1039/C5AY01071C. Epub 2015 Aug 20. PMID: 26527562; PMCID: PMC4625556.
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Anal Methods. 2015 Oct 21;7(20):8632-8639. doi: 10.1039/C5AY01071C. Epub 2015 Aug 20.

Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay.

Analytical methods : advancing methods and applications

Christine F Woolley, Mark A Hayes

Affiliations

  1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.

PMID: 26527562 PMCID: PMC4625556 DOI: 10.1039/C5AY01071C

Abstract

To achieve improved sensitivity in cardiac biomarker detection, a batch incubation magnetic microbead immunoassay was developed and tested on three separate human protein targets: myoglobin, heart-type fatty acid binding protein, and cardiac troponin I. A sandwich immunoassay was performed in a simple micro-centrifuge tube allowing full dispersal of the solid capture surface during incubations. Following magnetic bead capture and wash steps, samples were analyzed in the presence of a manipulated magnetic field utilizing a modified microscope slide and fluorescent inverted microscope to collect video data files. Analysis of the video data allowed for the quantitation of myoglobin, heart-type fatty acid binding protein and cardiac troponin I to levels of 360 aM, 67 fM, and 42 fM, respectively. Compared to the previous detection limit of 50 pM for myoglobin, this offers a five-fold improvement in sensitivity. This improvement in sensitivity and incorporation of additional markers, along with the small sample volumes required, suggest the potential of this platform for incorporation as a detection method in a total sample analysis device enabling multiplexed detection for the analysis of clinical samples.

Keywords: Cardiac biomarker; assay optimization; micro-immunoassay; microbead immunoassay; quantitation limit; sandwich immunoassay

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