Display options
Cite
Traylen C, Ramasubramanyan S, Zuo J, et al. Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells. Pathogens. 2015;4(4):739-51doi: 10.3390/pathogens4040739.
Traylen, C., Ramasubramanyan, S., Zuo, J., Rowe, M., Almohammad, R., Heesom, K., Sweet, S. M., Matthews, D. A., & Sinclair, A. J. (2015). Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells. Pathogens (Basel, Switzerland), 4(4), 739-51. https://doi.org/10.3390/pathogens4040739
Traylen, Chris, et al. "Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells." Pathogens (Basel, Switzerland) vol. 4,4 (2015): 739-51. doi: https://doi.org/10.3390/pathogens4040739
Traylen C, Ramasubramanyan S, Zuo J, Rowe M, Almohammad R, Heesom K, Sweet SM, Matthews DA, Sinclair AJ. Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells. Pathogens. 2015 Oct 29;4(4):739-51. doi: 10.3390/pathogens4040739. PMID: 26529022; PMCID: PMC4693162.
Copy Download .nbib Format: NLM
Share it on

Pathogens. 2015 Oct 29;4(4):739-51. doi: 10.3390/pathogens4040739.

Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells.

Pathogens (Basel, Switzerland)

Chris Traylen, Sharada Ramasubramanyan, Jianmin Zuo, Martin Rowe, Rajaei Almohammad, Kate Heesom, Steve M M Sweet, David A Matthews, Alison J Sinclair

Affiliations

  1. School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK. [email protected].
  2. School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK. [email protected].
  3. School of Cancer Sciences and Centre for Human Virology, University of Birmingham College of Medical and Dental Sciences, Edgbaston, Birmingham B15 2TT, UK. [email protected].
  4. School of Cancer Sciences and Centre for Human Virology, University of Birmingham College of Medical and Dental Sciences, Edgbaston, Birmingham B15 2TT, UK. [email protected].
  5. School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK. [email protected].
  6. School of Cellular and Molecular Medicine, University of Bristol, Medical Sciences Building, Bristol BS8 1TD, UK. [email protected].
  7. Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK. [email protected].
  8. School of Cellular and Molecular Medicine, University of Bristol, Medical Sciences Building, Bristol BS8 1TD, UK. [email protected].
  9. School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK. [email protected].

PMID: 26529022 PMCID: PMC4693162 DOI: 10.3390/pathogens4040739

Abstract

The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.

Keywords: Epstein-Barr; cancer; herpes; proteome; replication; virus

References

  1. Nat Methods. 2012 Dec;9(12):1207-11 - PubMed
  2. Nucleic Acids Res. 2015 Apr 20;43(7):3563-77 - PubMed
  3. EMBO J. 2000 Jun 15;19(12):3080-9 - PubMed
  4. J Virol. 2006 May;80(10):5078-81 - PubMed
  5. Sheng Li Xue Bao. 2014 Feb 25;66(1):79-84 - PubMed
  6. Ann Oncol. 2010 Oct;21 Suppl 7:vii308-12 - PubMed
  7. PLoS Pathog. 2014 Aug 28;10(8):e1004307 - PubMed
  8. J Virol. 2008 Apr;82(8):4042-51 - PubMed
  9. J Virol. 2000 Nov;74(21):10142-52 - PubMed
  10. Int J Oncol. 2015 Apr;46(4):1421-34 - PubMed
  11. J Virol. 1991 Jun;65(6):2868-74 - PubMed
  12. Rev Med Virol. 2013 Jul;23(4):213-20 - PubMed
  13. PLoS Biol. 2011 May;9(5):e1001074 - PubMed
  14. Semin Cancer Biol. 2009 Dec;19(6):377-88 - PubMed
  15. J Virol. 2014 Feb;88(3):1604-16 - PubMed
  16. Virology. 2015 Jan 1;474:117-30 - PubMed
  17. J Virol. 2002 Oct;76(19):9635-44 - PubMed
  18. Blood. 2005 Jul 1;106(1):235-40 - PubMed
  19. Virology. 2009 Jun 20;389(1-2):75-81 - PubMed
  20. J Virol. 2009 May;83(9):4616-23 - PubMed
  21. Chin J Cancer. 2014 Dec;33(12):591-7 - PubMed
  22. PLoS Pathog. 2009 Jun;5(6):e1000490 - PubMed
  23. J Virol. 2012 Feb;86(3):1458-67 - PubMed
  24. J Virol. 1998 Mar;72(3):2463-73 - PubMed
  25. J Virol. 2013 Feb;87(4):2011-22 - PubMed
  26. J Virol. 1995 May;69(5):2998-3006 - PubMed
  27. J Virol. 2009 Nov;83(21):10877-91 - PubMed
  28. Nat Methods. 2015 Jul;12(7):605-8 - PubMed
  29. J Virol. 2006 Oct;80(19):9435-43 - PubMed
  30. Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16286-91 - PubMed
  31. J Virol. 1992 Aug;66(8):5030-9 - PubMed
  32. Nat Biotechnol. 2014 Mar;32(3):223-6 - PubMed
  33. PLoS Pathog. 2011 Dec;7(12):e1002455 - PubMed
  34. Nature. 1984 Jul 19-25;310(5974):207-11 - PubMed
  35. J Virol. 2009 May;83(10):4952-62 - PubMed
  36. Lancet. 1964 Mar 28;1(7335):702-3 - PubMed
  37. J Gen Virol. 2008 Apr;89(Pt 4):884-95 - PubMed
  38. PLoS Pathog. 2013;9(5):e1003341 - PubMed
  39. BMC Genomics. 2011 Dec 20;12:625 - PubMed
  40. Medicine (Baltimore). 2015 May;94(20):e792 - PubMed
  41. Nat Rev Cancer. 2009 Jan;9(1):15-27 - PubMed
  42. J Virol. 2005 Mar;79(6):3703-12 - PubMed
  43. J Virol. 2015 Mar;89(6):3112-22 - PubMed
  44. J Virol. 2015 May;89(9):5002-11 - PubMed

Publication Types

Grant support