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Erdinest N, Shohat N, Moallem E, et al. Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid. J Inflamm (Lond). 2015;12:59doi: 10.1186/s12950-015-0104-1.
Erdinest, N., Shohat, N., Moallem, E., Yahalom, C., Mechoulam, H., Anteby, I., Ovadia, H., & Solomon, A. (2015). Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid. Journal of inflammation (London, England), 1259. https://doi.org/10.1186/s12950-015-0104-1
Erdinest, Nir, et al. "Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid." Journal of inflammation (London, England) vol. 12 (2015): 59. doi: https://doi.org/10.1186/s12950-015-0104-1
Erdinest N, Shohat N, Moallem E, Yahalom C, Mechoulam H, Anteby I, Ovadia H, Solomon A. Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid. J Inflamm (Lond). 2015 Oct 25;12:59. doi: 10.1186/s12950-015-0104-1. eCollection 2015. PMID: 26500455; PMCID: PMC4619984.
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J Inflamm (Lond). 2015 Oct 25;12:59. doi: 10.1186/s12950-015-0104-1. eCollection 2015.

Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid.

Journal of inflammation (London, England)

Nir Erdinest, Noam Shohat, Eli Moallem, Claudia Yahalom, Hadas Mechoulam, Irene Anteby, Haim Ovadia, Abraham Solomon

Affiliations

  1. Departments of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  2. Clinical Immunology and Allergy Unit, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  3. Department of Neurology at The Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  4. Cornea and Refractive Surgery Service, Department of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem, 91120 Israel.

PMID: 26500455 PMCID: PMC4619984 DOI: 10.1186/s12950-015-0104-1

Abstract

PURPOSE: It is known that both human conjunctival fibroblasts (HCF) and corneal epithelial (HCE) cells contribute to the inflammatory process in the ocular surface by releasing inflammatory cytokines. In addition, nitric oxide (NO) has an important role in inflammatory responses in the ocular surface. In the present study, we aimed to characterize the capacity of these cells to release nitric oxide in response to cytokines and Lipopolysaccharide (LPS), and show that Alpha-linoleic acid (ALA) inhibits these responses.

METHODS: HCF, HCE cells, peripheral blood mononuclear cells (PBMCs) and co-culture of HCF and PBMC were treated with different combinations of inflammatory inducers, including interleukin)IL- (6, tumor necrosis factors (TNF)-α, interferon (IFN)- γ and IL-1β and LPS. Nitrite levels were measured in cell supernatants with and without ALA by the Griess reaction test at 24, 48 and 72 h respectively. Expression of nitric oxide synthase 2 (NOS-2) was evaluated by real-time PCR.

RESULTS: All cytokine combinations had an inducible effect on nitrite secretion in HCF, PBMC and co-cultured PBMC and HCF, but not in HCE cells. Treatment with a combination of IL-6, LPS, TNF-α, IFN- γ and IL-1β induced the highest nitrite secretion (2.91 fold, P < 0.01) as compared to cells incubated in medium alone. nitrite secretion was reduced by 38.9 % (P < 0.05) after treatment with ALA alone. Co-culturing PBMC with HCF with and without ALA treatment demonstrated similar results in nitrite level as,compared to PBMC alone. In addition, ALA significantly decreased NOS-2 expression in HCF by 48.9 % (P < 0. 001) after 72 h.

CONCLUSIONS: The decrease in nitrite release and inhibition of NOS-2 expression indicate that ALA may have an anti-inflammatory effect both on HCF and on peripheral immune cells. This indicates that ALA may serve as a potent anti-inflammatory agent in ocular surface inflammation.

Keywords: Alpha linolenic acid; Conjunctival fibroblasts; Corneal epithelium; Nitric oxide

References

  1. J Am Coll Nutr. 2002 Dec;21(6):495-505 - PubMed
  2. Curr Opin Immunol. 2000 Feb;12(1):64-76 - PubMed
  3. Exp Eye Res. 1998 Dec;67(6):619-24 - PubMed
  4. Eur J Ophthalmol. 1998 Jul-Sep;8(3):137-41 - PubMed
  5. Biomed Pharmacother. 2002 Oct;56(8):365-79 - PubMed
  6. Exp Eye Res. 2001 Oct;73(4):449-59 - PubMed
  7. Biochem Biophys Res Commun. 1995 Oct 4;215(1):386-93 - PubMed
  8. Nitric Oxide. 1997 Oct;1(5):397-403 - PubMed
  9. Cell. 1994 Sep 23;78(6):915-8 - PubMed
  10. Genome Res. 1996 Oct;6(10):986-94 - PubMed
  11. Int Immunopharmacol. 2001 Aug;1(8):1491-500 - PubMed
  12. Invest Ophthalmol Vis Sci. 1983 Aug;24(8):1139-43 - PubMed
  13. Evid Rep Technol Assess (Summ). 2005 Aug;(118):1-11 - PubMed
  14. J Natl Cancer Inst. 1983 Jun;70(6):1005-10 - PubMed
  15. Invest Ophthalmol Vis Sci. 1997 Sep;38(10):2045-52 - PubMed
  16. Int Immunopharmacol. 2001 Aug;1(8):1457-67 - PubMed
  17. Invest Ophthalmol Vis Sci. 2005 Dec;46(12):4474-9 - PubMed
  18. Lancet. 1979 Sep 1;2(8140):433-5 - PubMed
  19. Genome Res. 1996 Oct;6(10):995-1001 - PubMed
  20. J Korean Med Sci. 2002 Jun;17(3):389-94 - PubMed
  21. Cont Lens Anterior Eye. 2008 Jun;31(3):141-6; quiz 170 - PubMed
  22. Arterioscler Thromb. 1994 Nov;14(11):1829-36 - PubMed
  23. Br J Ophthalmol. 1995 Jun;79(6):590-5 - PubMed
  24. Arch Ophthalmol. 2008 Feb;126(2):219-25 - PubMed
  25. Anal Biochem. 1982 Oct;126(1):131-8 - PubMed
  26. Pharmacol Rev. 1991 Jun;43(2):109-42 - PubMed
  27. FASEB J. 1992 Sep;6(12):3051-64 - PubMed
  28. Lipids Health Dis. 2008 Oct 15;7:37 - PubMed
  29. J Clin Invest. 1981 Sep;68(3):621-30 - PubMed
  30. Invest Ophthalmol Vis Sci. 1999 Nov;40(12):2773-9 - PubMed
  31. Biochem Biophys Res Commun. 1996 Mar 27;220(3):618-22 - PubMed
  32. Allergy Asthma Proc. 1998 Nov-Dec;19(6):349-51 - PubMed
  33. J Immunol. 1994 Apr 15;152(8):4110-8 - PubMed
  34. Invest Ophthalmol Vis Sci. 2001 Mar;42(3):713-9 - PubMed
  35. N Engl J Med. 1988 Mar 3;318(9):549-57 - PubMed
  36. J Biol Chem. 1994 Feb 18;269(7):4705-8 - PubMed
  37. Invest Ophthalmol Vis Sci. 1994 Mar;35(3):1107-13 - PubMed
  38. Invest Ophthalmol Vis Sci. 2012 Jul 03;53(8):4396-406 - PubMed
  39. Invest Ophthalmol Vis Sci. 1996 Apr;37(5):826-32 - PubMed
  40. Cornea. 2000 Jan;19(1):84-91 - PubMed

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