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Pini NI, Lima DA, Ambrosano GM, et al. Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis. J Clin Exp Dent. 2015;7(4):e506-12doi: 10.4317/jced.51416.
Pini, N. I., Lima, D. A., Ambrosano, G. M., da Silva, W. J., Aguiar, F. H., & Lovadino, J. R. (2015). Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis. Journal of clinical and experimental dentistry, 7(4), e506-12. https://doi.org/10.4317/jced.51416
Pini, Núbia-Inocencya-Pavesi, et al. "Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis." Journal of clinical and experimental dentistry vol. 7,4 (2015): e506-12. doi: https://doi.org/10.4317/jced.51416
Pini NI, Lima DA, Ambrosano GM, da Silva WJ, Aguiar FH, Lovadino JR. Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis. J Clin Exp Dent. 2015 Oct 01;7(4):e506-12. doi: 10.4317/jced.51416. eCollection 2015 Oct. PMID: 26535098; PMCID: PMC4628806.
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J Clin Exp Dent. 2015 Oct 01;7(4):e506-12. doi: 10.4317/jced.51416. eCollection 2015 Oct.

Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis.

Journal of clinical and experimental dentistry

Núbia-Inocencya-Pavesi Pini, Débora-Alves-Nunes-Leite Lima, Gláucia-Maria-Bovi Ambrosano, Wander-José da Silva, Flávio-Henrique-Baggio Aguiar, José-Roberto Lovadino

Affiliations

  1. PhD applicant in Restorative Dentistry, Piracicaba Dental School, University of Campinas - FOP/Unicamp.
  2. Professor, Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas - FOP/Unicamp.
  3. Professor, Department of Social Dentistry/Statistics, Piracicaba Dental School, State University of Campinas, Piracicaba-SP/Brazil.
  4. Professor, Department of Prosthesis, Piracicaba Dental School, State University of Campinas, Piracicaba-SP/Brazil.

PMID: 26535098 PMCID: PMC4628806 DOI: 10.4317/jced.51416

Abstract

BACKGROUND: This study evaluated the effects of the acids used in the microabrasion on enamel.

MATERIAL AND METHODS: Seventy enamel/dentine blocks (25 mm2) of bovine incisors were divided into 7 groups (n=10). Experimental groups were treated by active/passive application of 35% H3PO4 (E1/E2) or 6.6% HCl (E3/E4). Control groups were treated by microabrasion with H3PO4+pumice (C5), HCl+silica (C6), or no treatment (C7). The superficial (SMH) and cross-sectional (CSMH; depths of 10, 25, 50, and 75 µm) microhardness of enamel were analyzed. Morphology was evaluated by confocal laser-scanning microscopy (CLSM). Data were analyzed by analysis of variance (Proc Mixed), Tukey, and Dunnet tests (α=5%).

RESULTS: Active application (E1 and E3) resulted in higher microhardness than passive application (E2 and E4), with no difference between acids. For most groups, the CSMH decreased as the depth increased. All experimental groups and negative controls (C5 and C6) showed significantly reduced CSMH values compared to the control. A significantly higher mean CSMH result was obtained with the active application of H3PO4 (E1) compared to HCl (E3). Passive application did not result in CSMH differences between acids. CLSM revealed the conditioning pattern for each group.

CONCLUSIONS: Although the acids displayed an erosive action, use of microabrasive mixture led to less damage to the enamel layers.

KEY WORDS: Enamel microabrasion, enamel microhardness, confocal laser scanning microscopy.

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