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Dudele A, Fischer CW, Elfving B, et al. Chronic exposure to low doses of lipopolysaccharide and high-fat feeding increases body mass without affecting glucose tolerance in female rats. Physiol Rep. 2015;3(11)doi: 10.14814/phy2.12584.
Dudele, A., Fischer, C. W., Elfving, B., Wegener, G., Wang, T., & Lund, S. (2015). Chronic exposure to low doses of lipopolysaccharide and high-fat feeding increases body mass without affecting glucose tolerance in female rats. Physiological reports, 3(11), . https://doi.org/10.14814/phy2.12584
Dudele, Anete, et al. "Chronic exposure to low doses of lipopolysaccharide and high-fat feeding increases body mass without affecting glucose tolerance in female rats." Physiological reports vol. 3,11 (2015). doi: https://doi.org/10.14814/phy2.12584
Dudele A, Fischer CW, Elfving B, Wegener G, Wang T, Lund S. Chronic exposure to low doses of lipopolysaccharide and high-fat feeding increases body mass without affecting glucose tolerance in female rats. Physiol Rep. 2015 Nov;3(11). doi: 10.14814/phy2.12584. PMID: 26537342; PMCID: PMC4673625.
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Physiol Rep. 2015 Nov;3(11). doi: 10.14814/phy2.12584.

Chronic exposure to low doses of lipopolysaccharide and high-fat feeding increases body mass without affecting glucose tolerance in female rats.

Physiological reports

Anete Dudele, Christina W Fischer, Betina Elfving, Gregers Wegener, Tobias Wang, Sten Lund

Affiliations

  1. Section for Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark [email protected].
  2. Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark.
  3. Section for Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark.
  4. Medical Research Laboratory, Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.

PMID: 26537342 PMCID: PMC4673625 DOI: 10.14814/phy2.12584

Abstract

Obesity-related inflammation may have a causal role in the development of diabetes and insulin resistance, and studies using animal models of chronic experimental endotoxemia have shown the link. However, many studies use only males, and much less is known about the role of obesity-related inflammation in females. Therefore, we addressed how experimentally induced chronic inflammation affects body mass, energy intake, and glucose metabolism in female rats. Adult female Sprague Dawley rats were instrumented with slow release pellets that delivered a constant daily dose of 53 or 207 μg of lipopolysaccharide (LPS) per rat for 60 days. Control rats were instrumented with vehicle pellets. Due to inflammatory nature of high-fat diet (HFD) half of the rats received HFD (60% of calories from lard), while the other half remained on control diet to detect possible interactions between two modes of induced inflammation. Our results showed that chronic LPS administration increased female rat body mass and calorie intake in a dose-dependent manner, and that HFD further exacerbated these effects. Despite these effects, no effects of LPS and HFD were evident on female rat glucose metabolism. Only LPS elevated expression of inflammatory markers in the hypothalamus. To conclude, female rats respond to experimentally induced chronic inflammation by increasing body mass, but do not develop glucose intolerance in the given period of time.

© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Keywords: Chronic endotoxemia; female rats; glucose tolerance; high‐fat diet

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