Biomater Res. 2015 Oct 26;19:21. doi: 10.1186/s40824-015-0042-2. eCollection 2015.
Encapsulation of pancreatic islet with HMGB1 fragment for attenuating inflammation.
Biomaterials research
Eun Hee Jo, Yong Hwa Hwang, Dong Yun Lee
Affiliations
Affiliations
- Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-791 Republic of Korea.
- BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791 Republic of Korea.
PMID: 26504589
PMCID: PMC4620643 DOI: 10.1186/s40824-015-0042-2
Abstract
BACKGROUND: Pancreatic islet encapsulation is one way to address the disadvantages of islet transplantation. Not only does encapsulation involve bidirectional diffusion of nutrients, oxygen, and glucose, but also it protects the graft from the recipient's immune reaction. The high mobility group box 1 (HMGB1), one of higher expression proteins in islet, can be secreted from transplanted islets and induce the inflammation. Therefore, the regulation of HMGB1-mediated inflammation is very important for successful islet transplantation. In this study, we used the HMGB1 A box, an antagonist of HMGB1 receptor in the immune cells, in the encapsulation of isolated islets as a new strategy.
RESULT: For co-encapsulation of HMGB1 A box protein with islets, we evaluated the distribution of alginate bead diameter. The average diameter of empty alginate bead was similar to that of alginate bead with islets. When different concentrations of HMGB1 A box protein was co-encapsulated with islets, it did not affect the viability and insulin secretion function of the islets. When the alginate beads with islets plus HMGB1 A box protein were cultured with macrophage, the amount of TNF-α secreted from the macrophages was significantly attenuated when compared to cultivation of unencapsulated islets or encapsulated islets. When the alginate beads with islets plus HMGB1 A box protein were intraperitoneally xenotransplanted into the diabetic mice, the survival rate of the islets was strongly improved with 2-fold.
CONCLUSION: Collectively, these results suggested that the encapsulation of HMGB1 A box protein might offer a protective effect in islet transplantation.
Keywords: Alginate bead; Encapsulation; HMGB1 A box; High mobility group box 1 (HMGB1); Pancreatic islet transplantation
References
- Transplant Proc. 2011 Nov;43(9):3156-60 - PubMed
- Eur J Immunol. 2004 Jun;34(6):1503-12 - PubMed
- Curr Opin Endocrinol Diabetes Obes. 2007 Apr;14(2):146-50 - PubMed
- Nat Rev Immunol. 2005 Apr;5(4):331-42 - PubMed
- J Endotoxin Res. 2002;8(6):469-72 - PubMed
- Science. 1980 Nov 21;210(4472):908-10 - PubMed
- World J Surg. 2001 Apr;25(4):481-6 - PubMed
- Nat Med. 2007 Apr;13(4):390-1 - PubMed
- J Diabetes Res. 2013;2013:374925 - PubMed
- Diabetologia. 2002 Feb;45(2):159-73 - PubMed
- N Engl J Med. 2000 Jul 27;343(4):230-8 - PubMed
- Diabetes Technol Ther. 2005 Dec;7(6):968-85 - PubMed
- Nat Rev Immunol. 2004 Apr;4(4):259-68 - PubMed
- Surgery. 1967 Jun;61(6):827-37 - PubMed
- Expert Opin Biol Ther. 2002 Jun;2(5):503-11 - PubMed
- Nat Rev Rheumatol. 2012 Jan 31;8(4):195-202 - PubMed
- J Clin Invest. 2010 Mar;120(3):735-43 - PubMed
- Cell Transplant. 2003;12 (8):867-75 - PubMed
Publication Types