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Wade JH, Alsop AT, Vertin NR, et al. Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays. ACS Cent Sci. 2015;1(7):374-382doi: 10.1021/acscentsci.5b00250.
Wade, J. H., Alsop, A. T., Vertin, N. R., Yang, H., Johnson, M. D., & Bailey, R. C. (2015). Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays. ACS central science, 1(7), 374-382. https://doi.org/10.1021/acscentsci.5b00250
Wade, James H, et al. "Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays." ACS central science vol. 1,7 (2015): 374-382. doi: https://doi.org/10.1021/acscentsci.5b00250
Wade JH, Alsop AT, Vertin NR, Yang H, Johnson MD, Bailey RC. Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays. ACS Cent Sci. 2015 Oct 28;1(7):374-382. doi: 10.1021/acscentsci.5b00250. Epub 2015 Sep 30. PMID: 26539563; PMCID: PMC4626792.
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ACS Cent Sci. 2015 Oct 28;1(7):374-382. doi: 10.1021/acscentsci.5b00250. Epub 2015 Sep 30.

Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays.

ACS central science

James H Wade, Aurora T Alsop, Nicholas R Vertin, Hongwei Yang, Mark D Johnson, Ryan C Bailey

Affiliations

  1. Department of Chemistry, University of Illinois at Urbana-Champaign , 600 South Mathews Avenue, Urbana, Illinois 61801, United States.
  2. Department of Neurological Surgery, Brigham and Women's Hospital and Harvard Medical School , Boston, Massachusetts 02115, United States.

PMID: 26539563 PMCID: PMC4626792 DOI: 10.1021/acscentsci.5b00250

Abstract

Extracellular signaling is commonly mediated through post-translational protein modifications that propagate messages from membrane-bound receptors to ultimately regulate gene expression. Signaling cascades are ubiquitously intertwined, and a full understanding of function can only be gleaned by observing dynamics across multiple key signaling nodes. Importantly, targets within signaling cascades often represent opportunities for therapeutic development or can serve as diagnostic biomarkers. Protein phosphorylation is a particularly important post-translational modification that controls many essential cellular signaling pathways. Not surprisingly, aberrant phosphorylation is found in many human diseases, including cancer, and phosphoprotein-based biomarker signatures hold unrealized promise for disease monitoring. Moreover, phosphoprotein analysis has wide-ranging applications across fundamental chemical biology, as many drug discovery efforts seek to target nodes within kinase signaling pathways. For both fundamental and translational applications, the analysis of phosphoprotein biomarker targets is limited by a reliance on labor-intensive and/or technically challenging methods, particularly when considering the simultaneous monitoring of multiplexed panels of phosphoprotein biomarkers. We have developed a technology based upon arrays of silicon photonic microring resonator sensors that fills this void, facilitating the rapid and automated analysis of multiple phosphoprotein levels from both cell lines and primary human tumor samples requiring only minimal sample preparation.

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