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Bello A, Daru BH, Stirton CH, et al. DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera. AoB Plants. 2015;7doi: 10.1093/aobpla/plv124.
Bello, A., Daru, B. H., Stirton, C. H., Chimphango, S. B., van der Bank, M., Maurin, O., & Muasya, A. M. (2015). DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera. AoB PLANTS, 7. https://doi.org/10.1093/aobpla/plv124
Bello, Abubakar, et al. "DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera." AoB PLANTS vol. 7 (2015). doi: https://doi.org/10.1093/aobpla/plv124
Bello A, Daru BH, Stirton CH, Chimphango SB, van der Bank M, Maurin O, Muasya AM. DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera. AoB Plants. 2015 Oct 27;7. doi: 10.1093/aobpla/plv124. PMID: 26507570; PMCID: PMC4670488.
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AoB Plants. 2015 Oct 27;7. doi: 10.1093/aobpla/plv124.

DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera.

AoB PLANTS

Abubakar Bello, Barnabas H Daru, Charles H Stirton, Samson B M Chimphango, Michelle van der Bank, Olivier Maurin, A Muthama Muasya

Affiliations

  1. Bolus Herbarium, Biological Sciences Department, University of Cape Town, Private Bag X3, Rondebosch 7700, South Africa [email protected].
  2. Department of Plant Science, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa.
  3. Bolus Herbarium, Biological Sciences Department, University of Cape Town, Private Bag X3, Rondebosch 7700, South Africa.
  4. African Centre for DNA Barcoding, Department of Botany and Plant Biotechnology, University of Johannesburg, PO Box 524, Auckland Park 2006, Johannesburg, South Africa.

PMID: 26507570 PMCID: PMC4670488 DOI: 10.1093/aobpla/plv124

Abstract

Large-scale DNA barcoding provides a new technique for species identification and evaluation of relationships across various levels (populations and species) and may reveal fundamental processes in recently diverged species. Here, we analysed DNA sequence variation in the recently diverged legumes from the Psoraleeae (Fabaceae) occurring in the Cape Floristic Region (CFR) of southern Africa to test the utility of DNA barcodes in species identification and discrimination. We further explored the phylogenetic signal on fire response trait (reseeding and resprouting) at species and generic levels. We showed that Psoraleoid legumes of the CFR exhibit a barcoding gap yielding the combination of matK and rbcLa (matK + rbcLa) data set as a better barcode than single regions. We found a high score (100 %) of correct identification of individuals to their respective genera but a very low score (<50 %) in identifying them to species. We found a considerable match (54 %) between genetic species and morphologically delimited species. We also found that different lineages showed a weak but significant phylogenetic conservatism in their response to fire as reseeders or resprouters, with more clustering of resprouters than would be expected by chance. These novel microevolutionary patterns might be acting continuously over time to produce multi-scale regularities of biodiversity. This study provides the first insight into the DNA barcoding campaign of land plants in species identification and detection of the phylogenetic signal in recently diverged lineages of the CFR.

Published by Oxford University Press on behalf of the Annals of Botany Company.

Keywords: Fabaceae; Otholobium; Psoralea; South Africa; reseeders; resprouters

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